Advertisement

Science in China Series C: Life Sciences

, Volume 40, Issue 5, pp 502–511 | Cite as

Synaptic organization of substance P, glutamate and GABA-immunoreactive boutons on functionally identified neurons in cat spinal deeper dorsal horn

  • Feng Wei
  • Zhiqi Zhao
Article
  • 16 Downloads

Abstract

In order to determine how nociceptive input conveyed by the C-fibers terminating in superficial laminae of the spinal cord reaches the wide dynamic range (WDR) cells in deeper dorsal horn, which functions as ascending projection pathway, the morphological features of some WDR cells in the deeper dorsal horn of the cat lumbar spinal cord were studied by intracellular injection of horseradish peroxidase and physiological characterization. One of the fully stained neurons with somata in lamina V and dendrites that entered lamina II were examined by electron microscopy. Immunogold staining of ultrathin sections through the labeled proximal dendrites in lamina II revealed that these dendrites received numerous synapses from substance P and glutamate immunoreactive (IR) axons, which were considered originating from C-fibers. In addition, many GABA-IR terminals were found presynaptic to the labeled dendrites. The results, therefore, suggest that the information carried by primary afferent can be sent from the superficial dorsal horn to the deeper laminae through monosynaptic contacts between C-fiber terminals and the long dorsal dendrites of some WDR cells in the deeper laminae, and that GABAergic system is involved in postsynaptic control to modulate the transmission of nociceptive sensory information.

Keywords

GABA glutamate immunoelectron microscopy intracellular staining substance P spinal cord wide dynamic range neuron 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Willis, W. D., Coggeshall, R. E.,Sensory Mechunisms of the Spinal Curd, New York: Plenum Press, 1991.Google Scholar
  2. 2.
    Woolf, C. J., King, A. E., Physiology and morphology of multireceptive neurons with C-afferent inputs in the deep dorsal horn of the rat lumbar spinal cord,J. Neurophysiol., 1987, 58: 460.PubMedGoogle Scholar
  3. 3.
    Ritz, L., Greenspan, J. D., Morphological features of lamina V neurons receiving nociceptive input in cat sacrocaudal spinal cord,J. Comp. Neurol., 1985, 238: 440.CrossRefPubMedGoogle Scholar
  4. 4.
    Koninck, Y. D., Ribedo-Silva, A., Henry, J. L.et al., Spinal neurons exhibiting a specific nociceptive response receive abundant substance P-containing synaptic contacts,Proc. Natl. Acad. Sci. USA, 1992, 89: 5073.CrossRefPubMedGoogle Scholar
  5. 5.
    Kangrga, I., Jiang, M., Randic, M., Actions of (-)-badofen on rat dorsal horn neurons,Bruin Res., 1991, 562: 265.CrossRefGoogle Scholar
  6. 6.
    Yang, H. Q., Zhao, Z. Q., Zhang, K. M., Facilitation of nociceptive responses in spinal dorsal horn neurones by blockade of myelinated afferent fibers,Chinese J. Physiol. Sci., 1990, 6(1): 47.Google Scholar
  7. 7.
    Wei, F., Zhao, Z. Q., Qian, X. Z., Synaptic organization of substance P, glutamate and CABA-immunoreactive boutons on functionally identified spinal dorsal horn neurons in the cat,Abstract of the Fourth IBRO World Congress of Neuroscience, Oxford: Rapid Communication of Oxford Ltd., 1995, 306.Google Scholar
  8. 8.
    Ottersen, O. P., Quantitative electron microscopic immunocytochemistry of neuroactive amino acids,Anat. Embryol., 1989, 180: 1.CrossRefPubMedGoogle Scholar
  9. 9.
    Todd, A. J., Cell in laminae III and IV of rat spinal dorsal horn receive monosynaptic primary afferent input in lamina II,J. Comp. Neurol., 1989, 289: 676.CrossRefPubMedGoogle Scholar
  10. 10.
    Westlund, K. N., Carlton, S. M., Zhang, D.et al., Glutamate-immunoreactive terminals synapse on primate spinothalamic tract cells,J. Comp. Neurol., 1992, 322: 519.CrossRefPubMedGoogle Scholar
  11. 11.
    Carlton, S. M., Westlund, K. N., Zhang, D.et al., Calcitonin gene-related peptide containing primary afferent fibers synapse on primate spinothalamic tract cells,Neurosci. Lett., 1990, 109: 76.CrossRefPubMedGoogle Scholar
  12. 12.
    Maxwell, D. J., Christie, W. M., Short, A. D.et al., Central boutons of glomeruli in the spinal cord of the cat are enriched with L-glutamate-like immunoreactivity,Neurosci., 1990, 36: 83.CrossRefGoogle Scholar
  13. 13.
    Zhao, Z. Q., Yang, H. Q., Zhang, K. M.et al., Release and depletion of substance P by capsaicin in substantia gelatinosa studied with antibody microprobe and immunohistochemistry,Neuropeptides, 1992, 23: 161.CrossRefPubMedGoogle Scholar
  14. 14.
    Song, X. J., Zhao, Z. Q., Interaction between substance P and excitatory amino acid receptors in modulation of nociceptive responses of cat spinal dorsal horn neurons,Neurmi. Lett., 1994, 168: 49.CrossRefGoogle Scholar
  15. 15.
    Carlton, S. M., Westlund, K. N., Zhang, D.et al., GABA-immunoreactive terminals synapse on primate spinothalamic tract cells,J. Comp. Neurol., 1992, 322: 528.CrossRefPubMedGoogle Scholar

Copyright information

© Science in China Press 1997

Authors and Affiliations

  • Feng Wei
    • 1
  • Zhiqi Zhao
    • 1
  1. 1.Shanghai Brain Research InstituteChinese Academy of SciencesShanghaiChina

Personalised recommendations