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Chinese Science Bulletin

, Volume 48, Issue 5, pp 465–468 | Cite as

PCR-RFLP analysis of mitochondrial DNA ND5/6 region among 3 subspecies of common carp (Cyprinus carpio L.) and its application to genetic discrimination of subspecies

  • Jianfeng Zhou
  • Zhongwei Wang
  • Yuzhen Ye
  • Qingjiang Wu
Reports

Abstract

Mitochondrial DNA ND5/6 region was studied by PCR-RFLP analysis among ten representative strains belonging to three subspecies (Cyprinus carpio carpio, Cyprinus carpio haematopterus andCyprinus carpio rubrofuscus) of common carp (Cyprinus carpio L.). A total of 2.4 kb fragment was amplified and subjected to restriction endonuclease analysis with nine restriction endonucleases subsequently. The results indicated that each subspecies owned one hyplotype and four restriction enzymes (Dde I, HaeIII,Taq I andMbo I) produced diagnostic restriction sites which could be used for discriminating the three subspecies and as molecular genetic markers for assistant selective breeding of common carp.

Keywords

Cyprinus carpio subspecies ND5/6 PCR-RFLP molecular marker 

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Copyright information

© Science in China Press 2003

Authors and Affiliations

  • Jianfeng Zhou
    • 1
  • Zhongwei Wang
    • 1
  • Yuzhen Ye
    • 1
  • Qingjiang Wu
    • 1
  1. 1.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of HydrobiologyChinese Academy of SciencesWuhanChina

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