Chinese Science Bulletin

, Volume 48, Issue 5, pp 465–468 | Cite as

PCR-RFLP analysis of mitochondrial DNA ND5/6 region among 3 subspecies of common carp (Cyprinus carpio L.) and its application to genetic discrimination of subspecies

  • Jianfeng Zhou
  • Zhongwei Wang
  • Yuzhen Ye
  • Qingjiang Wu


Mitochondrial DNA ND5/6 region was studied by PCR-RFLP analysis among ten representative strains belonging to three subspecies (Cyprinus carpio carpio, Cyprinus carpio haematopterus andCyprinus carpio rubrofuscus) of common carp (Cyprinus carpio L.). A total of 2.4 kb fragment was amplified and subjected to restriction endonuclease analysis with nine restriction endonucleases subsequently. The results indicated that each subspecies owned one hyplotype and four restriction enzymes (Dde I, HaeIII,Taq I andMbo I) produced diagnostic restriction sites which could be used for discriminating the three subspecies and as molecular genetic markers for assistant selective breeding of common carp.


Cyprinus carpio subspecies ND5/6 PCR-RFLP molecular marker 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Komen, J., Clones of common carp, Cyprinus carpio, New perspectives in fish research, PhD thesis, Wageningen: Wageningen Agricultural University, 1990.Google Scholar
  2. 2.
    Chiba, T., Kurihama, N., Kenichi, Y., Culture of common carp (in Japanese), Tokyo: Green Book House Publisher, 1966.Google Scholar
  3. 3.
    Wohlfarth, G., Common carp, in Evolution of Domesticated Animals (ed. Mason, I. L.), Lodon: Longman, 1984, 375–380.Google Scholar
  4. 4.
    Wu, X. W. et al., Monographs of Cyprininae in China (in Chiense), Shanghai: Shanghai People’s Publisher, 1977, 408–428.Google Scholar
  5. 5.
    Avise, J. C., Molecular Markers, Natural History and Evolution, New York: Chapman & Hall, 1994.Google Scholar
  6. 6.
    Sambrook, J., Fritsch, E. F., Maniatis, T., Molecular Cloning: A Laboratory Manual (2nd ed.), Now York: Cold Spring Harbor Laboratory Press, 1989.Google Scholar
  7. 7.
    Park, K. L., Brainard, M. A., Dightman, D. A. et al., Low levels of intraspecific variation in the mitochondrial DNA of chum salmo (Onchorhynchus keta), Mol. Mar. Biol. Biotechnol., 1993, 2: 263–270.Google Scholar
  8. 8.
    McElroy, D., Moran, P., Bermingham, E. et al., REAP: An integrated environment for the manipulation and phylogenetic analysis of restriction data, J. Hered., 1992, 83: 157–158.Google Scholar
  9. 9.
    Nei, M., Li, W. H., Mathematical model for studying genetic variant in terms of restriction endonucleases, Proc. Natl. Acad. Sci. USA, 1979, 76: 5269–5273.CrossRefGoogle Scholar
  10. 10.
    Nei, M., Molecular Evolutionary Genetics, New York: Columbia University Press, 1987.Google Scholar
  11. 11.
    Kumar, S., Tamura, K., Jakobsen, I. B., MEGA 2: Molecular evolutionary genetics analysis software, Tempe: Arizona State University, 2001.Google Scholar
  12. 12.
    Kohlmann, K., Kersten, P., Genetics variability of German and foreign common carp (Cyprinus carpio L.) population, Aquaculture, 1999, 173: 435–445.CrossRefGoogle Scholar
  13. 13.
    Gross, R., Kohlmann, K., Kersten, P., PCR-RFLP analysis of the mitochondrial DNA ND-3/4 and ND-5/6 gene polymorphisms in the European and East Asian subspecies of common carp (Cyprinus carpio L.), Aquaculture, 2002, 204: 507–516.CrossRefGoogle Scholar
  14. 14.
    Balon, E. K., Origin and domestication of the wild carp, Cyprinus carpio: from Roman gourmets to the swimming flowers, Aquaculture, 1995, 129: 3–47.CrossRefGoogle Scholar
  15. 15.
    Ojima, Y., Takai, A., Karytype study of colored-carp (Cyprinus carpio), a preliminary report, Proc. Jap. Acad., 1981, 57B: 7–12.CrossRefGoogle Scholar

Copyright information

© Science in China Press 2003

Authors and Affiliations

  • Jianfeng Zhou
    • 1
  • Zhongwei Wang
    • 1
  • Yuzhen Ye
    • 1
  • Qingjiang Wu
    • 1
  1. 1.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of HydrobiologyChinese Academy of SciencesWuhanChina

Personalised recommendations