Abstract
Mitochondrial DNA ND5/6 region was studied by PCR-RFLP analysis among ten representative strains belonging to three subspecies (Cyprinus carpio carpio, Cyprinus carpio haematopterus andCyprinus carpio rubrofuscus) of common carp (Cyprinus carpio L.). A total of 2.4 kb fragment was amplified and subjected to restriction endonuclease analysis with nine restriction endonucleases subsequently. The results indicated that each subspecies owned one hyplotype and four restriction enzymes (Dde I, HaeIII,Taq I andMbo I) produced diagnostic restriction sites which could be used for discriminating the three subspecies and as molecular genetic markers for assistant selective breeding of common carp.
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References
Komen, J., Clones of common carp, Cyprinus carpio, New perspectives in fish research, PhD thesis, Wageningen: Wageningen Agricultural University, 1990.
Chiba, T., Kurihama, N., Kenichi, Y., Culture of common carp (in Japanese), Tokyo: Green Book House Publisher, 1966.
Wohlfarth, G., Common carp, in Evolution of Domesticated Animals (ed. Mason, I. L.), Lodon: Longman, 1984, 375–380.
Wu, X. W. et al., Monographs of Cyprininae in China (in Chiense), Shanghai: Shanghai People’s Publisher, 1977, 408–428.
Avise, J. C., Molecular Markers, Natural History and Evolution, New York: Chapman & Hall, 1994.
Sambrook, J., Fritsch, E. F., Maniatis, T., Molecular Cloning: A Laboratory Manual (2nd ed.), Now York: Cold Spring Harbor Laboratory Press, 1989.
Park, K. L., Brainard, M. A., Dightman, D. A. et al., Low levels of intraspecific variation in the mitochondrial DNA of chum salmo (Onchorhynchus keta), Mol. Mar. Biol. Biotechnol., 1993, 2: 263–270.
McElroy, D., Moran, P., Bermingham, E. et al., REAP: An integrated environment for the manipulation and phylogenetic analysis of restriction data, J. Hered., 1992, 83: 157–158.
Nei, M., Li, W. H., Mathematical model for studying genetic variant in terms of restriction endonucleases, Proc. Natl. Acad. Sci. USA, 1979, 76: 5269–5273.
Nei, M., Molecular Evolutionary Genetics, New York: Columbia University Press, 1987.
Kumar, S., Tamura, K., Jakobsen, I. B., MEGA 2: Molecular evolutionary genetics analysis software, Tempe: Arizona State University, 2001.
Kohlmann, K., Kersten, P., Genetics variability of German and foreign common carp (Cyprinus carpio L.) population, Aquaculture, 1999, 173: 435–445.
Gross, R., Kohlmann, K., Kersten, P., PCR-RFLP analysis of the mitochondrial DNA ND-3/4 and ND-5/6 gene polymorphisms in the European and East Asian subspecies of common carp (Cyprinus carpio L.), Aquaculture, 2002, 204: 507–516.
Balon, E. K., Origin and domestication of the wild carp, Cyprinus carpio: from Roman gourmets to the swimming flowers, Aquaculture, 1995, 129: 3–47.
Ojima, Y., Takai, A., Karytype study of colored-carp (Cyprinus carpio), a preliminary report, Proc. Jap. Acad., 1981, 57B: 7–12.
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Zhou, J., Wang, Z., Ye, Y. et al. PCR-RFLP analysis of mitochondrial DNA ND5/6 region among 3 subspecies of common carp (Cyprinus carpio L.) and its application to genetic discrimination of subspecies. Chin.Sci.Bull. 48, 465–468 (2003). https://doi.org/10.1007/BF03183252
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DOI: https://doi.org/10.1007/BF03183252