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Chinese Science Bulletin

, Volume 48, Issue 4, pp 358–363 | Cite as

NO-mediated hypersensitive responses of rice suspension cultures induced by incompatible elicitor

  • Xiangyang Hu
  • S. J. Neill
  • Weiming Cai
  • Zhangcheng Tang
Reports

Abstract

Magnaporthe grisea special races 98-186-1G1 and 97-23-2D1 induce incompatible and compatible reactions respectively with rice “Xiushui”. The elicitor from the cell wall ofM. grisea race 98-186-1G1, termed IE, strongly induced the HR response in suspension cultures of rice “Xiushui”, including increased PAL activity, transcription ofpal, pr1, chi, cell death and the generation of NO. The elicitor prepared from the cell wall ofM. grisea race 97-23-2D1, named CE, was much less efficient at inducing such effects. The NOS enzyme inhibitors L-NAA and PBITU suppressed the production of NO induced by IE in “Xiushui” rice. The increased PAL activity and transcription ofpr1, pal, chi genes induced by IE were blocked by L-NAA, PBITU or CPTIO pretreatment. Direct treatment of rice cultures with the NO donor (SNP) also induced the transcription ofpr1,pal andchi genes. These data implicated that NO acted as a signal mediating the HR induced by IE in rice and showed that NO, in combination with H2O2, is necessary for induction of cell death by IE in rice suspension cells.

Keywords

NO elicitor HR rice 

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Copyright information

© Science in China Press 2003

Authors and Affiliations

  • Xiangyang Hu
    • 1
  • S. J. Neill
    • 2
  • Weiming Cai
    • 1
  • Zhangcheng Tang
    • 1
  1. 1.Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological SciencesChinese Academy of SciencesShanghaiChina
  2. 2.Centre for Research in Plant ScienceUniversity of the West of England, BristolBristolUK

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