Abstract
Magnaporthe grisea special races 98-186-1G1 and 97-23-2D1 induce incompatible and compatible reactions respectively with rice “Xiushui”. The elicitor from the cell wall ofM. grisea race 98-186-1G1, termed IE, strongly induced the HR response in suspension cultures of rice “Xiushui”, including increased PAL activity, transcription ofpal, pr1, chi, cell death and the generation of NO. The elicitor prepared from the cell wall ofM. grisea race 97-23-2D1, named CE, was much less efficient at inducing such effects. The NOS enzyme inhibitors L-NAA and PBITU suppressed the production of NO induced by IE in “Xiushui” rice. The increased PAL activity and transcription ofpr1, pal, chi genes induced by IE were blocked by L-NAA, PBITU or CPTIO pretreatment. Direct treatment of rice cultures with the NO donor (SNP) also induced the transcription ofpr1,pal andchi genes. These data implicated that NO acted as a signal mediating the HR induced by IE in rice and showed that NO, in combination with H2O2, is necessary for induction of cell death by IE in rice suspension cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Wang, J., The molecular mechanism of plant resistance to pathogens, Plant Molecular Physiology, 2nd ed., Beijing: Science Academy Press, 1998, 784–806.
Cai, Y. Y., Chen, J., The role of ROS in plant defense system, The Bulletin of Plant Sciences, 1999, 16(2): 107–112.
Guan, Y. Y., Lin, M. J., The role of NO in human immunity system, New Medicine, 1999, 30(1): 55–57.
Liang, W. S., Li, D. B., The role of NO in plant physiology and pathology, The Community of Plant Physiology, 2001, 37(6): 562–569.
Durner, J., Wendehenne, D., Klessig, D. F., Defense gene induction in tobacco by nitric oxide, cyclic CMP and cyclic ADP-ribose, Proc. Natl. Acad. Sci. USA, 1998, 95: 10328–10333.
Delledone, M., Xia, Y., Dixon, R. A. et al., Nitric oxide functions as a secondary signal in plant disease resistance, Nature, 1998, 394: 585–588.
Foissner, I., Wendehenne, D., Langebartels, C. et al., Technical advance:in vitro imaging of an elicitor-induced nitric oxide burst in tobacco, Plant J., 2000, 23: 817–824.
Neill, S., Desikan, R., Clarke, A. et al., Nitric oxide is a novel component of abscisic acid signaling in stomatal guard cells, Plant Physiol., 2002, 128: 13–16.
Kuchitsu, K., Kikuyama, M., Shibuya, N., N-acetyl-chitooligosaccharides, biotic elicitor for phytoalexin production, induce transient membrane depolarization in suspension-cultured rice cells, Protoplasma, 1993, 174: 79–81.
Ayers, A. R., Ebel, J., Valent, B. et al., Host-pathogen interactions X, fractionation and biological activity of an elicitor isolated from the mycelial walls ofPhytophthora megasperm var sojae, Plant Physiol., 1976, 57: 760–765.
Ding, A. H., Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophage, J. Immunol., 1998, 141: 2407–2412.
Shirasu, K., Nakajima, H., Rajasekhar, V. K. et al., Salicylic acid potentiates an agonist dependent gain control that amplifies pathogen signals in the activation of defense mechanisms, Plant Cell, 1997, 9: 261–270.
Grant, J. J., Loake, G. J., Role of reactive oxygen intermediates and cognate redox signaling in disease resistance. Plant Physiol., 2000, 124: 21–30.
Zihui, Y., Hailin, L., Tongyang, J., The molecular protocol of plant physiology, 2nd ed., Beijing: Science Press, 1988, 120–145.
Daoyue, S., Yanlin, G., Ligeng, M., The cell signal transduction, 2nd ed, Beijing: Science Press, 2000, 247–254.
Yamasaki, H., Sakihama, Y., Simultaneous production of nitric oxide and peroxynitrite by plant nitrate reductase:in vitro evidence for the NR-dependent formation of active nitrogen species, FEBS Letters, 2000, 468: 89–92.
Tang, M., Smith, J. C., Elicitor induced defense response in Medicago Sativa, New Physiologist, 2001, 149: 401–418.
Levine, A., Tenhaken, R., Dixon, R. et al., H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response, Cell, 1994, 79: 583–593.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Hu, X., Neill, S.J., Cai, W. et al. NO-mediated hypersensitive responses of rice suspension cultures induced by incompatible elicitor. Chin.Sci.Bull. 48, 358–363 (2003). https://doi.org/10.1007/BF03183230
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF03183230