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Chinese Science Bulletin

, Volume 48, Issue 4, pp 346–350 | Cite as

Physiological responses of osteoblasts to cyclic stretching and the change of intracellular calcium concentration

  • Liling Tang
  • Yuanliang Wang
  • Li Gu
  • Shaoxi Cai
  • Aihua Su
Reports

Abstract

The development of bone tissues is regulated by mechanical stimulation. Cyclic stretching was applied to the osteoblasts that were delivered from rat calvarie. The results showed that stretching at 500 με increased the cell proliferation while loading at 1000 με and 1500 με inhabited cell growth. Loading also increased the adhesive force between cells and substrate as well as spreading areas of osteobalsts. Furthermore, the fluorescence probe Fluo-3/AM was used to investigate the effect of stretching stimulation on the intracellular calcium concentration of osteoblasts. The intracellular calcium concentration of osteoblasts that were stretched at 500 με for 5 min was 92.9% higher than the control. After being treated with the panax ontoginseng saponins, the stretched osteoblasts still expressed 28.6% higher intracellular calcium concentration than that of the control, which proved that both the influx of extracellular calcium and the release of intracellular calcium store were involved in the increase of intracellular calcium concentration when osteoblasts responded to the cyclic stretching. And the influx of extracellular calcium through transmembrance channel played a main role.

Keywords

stretching osteoblasts proliferation adhesion intracellular calcium 

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Copyright information

© Science in China Press 2003

Authors and Affiliations

  • Liling Tang
    • 1
  • Yuanliang Wang
    • 1
  • Li Gu
    • 1
  • Shaoxi Cai
    • 1
  • Aihua Su
    • 1
  1. 1.Bio-engineering CollegeChongqing UniversityChongqingChina

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