Chinese Science Bulletin

, Volume 48, Issue 4, pp 346–350 | Cite as

Physiological responses of osteoblasts to cyclic stretching and the change of intracellular calcium concentration

  • Liling Tang
  • Yuanliang Wang
  • Li Gu
  • Shaoxi Cai
  • Aihua Su


The development of bone tissues is regulated by mechanical stimulation. Cyclic stretching was applied to the osteoblasts that were delivered from rat calvarie. The results showed that stretching at 500 με increased the cell proliferation while loading at 1000 με and 1500 με inhabited cell growth. Loading also increased the adhesive force between cells and substrate as well as spreading areas of osteobalsts. Furthermore, the fluorescence probe Fluo-3/AM was used to investigate the effect of stretching stimulation on the intracellular calcium concentration of osteoblasts. The intracellular calcium concentration of osteoblasts that were stretched at 500 με for 5 min was 92.9% higher than the control. After being treated with the panax ontoginseng saponins, the stretched osteoblasts still expressed 28.6% higher intracellular calcium concentration than that of the control, which proved that both the influx of extracellular calcium and the release of intracellular calcium store were involved in the increase of intracellular calcium concentration when osteoblasts responded to the cyclic stretching. And the influx of extracellular calcium through transmembrance channel played a main role.


stretching osteoblasts proliferation adhesion intracellular calcium 


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  1. 1.
    Karlsson, M. K., Johnell, O., Obrant, K. J., Is bone mineral density advantage maintained long-term in previous weight lifters? Calcif. Tissue Int., 1995, 57(5): 325–328.CrossRefGoogle Scholar
  2. 2.
    Turner, C. H., Forwood, M. R., Yoshikawa, T., Mechanical loading thresholds for lamellar and woven bone formation, J. Bone Miner Res., 1994, 9(1): 87–97.CrossRefGoogle Scholar
  3. 3.
    Kaspar, D., Seidl, W., Neidlinger-wilke, C. et al., Dynamic cell stretching increases human osteoblast proliferation and CICP synthesis but decreases osteocalcin synthesis and alkaline phosphatase activity, J. Biomechanics, 2000, 33(1): 45–51.CrossRefGoogle Scholar
  4. 4.
    Walker, L. M., Publicover, S. J., Preston, M. R. et al., Calcium channel activation and matrix protein upregulation in bone cells in response to mechanical strain, J. Cell Biochem., 2000, 79(4): 648–661.CrossRefGoogle Scholar
  5. 5.
    Ingber, D. E., How cells (might) sense microgravity, FASEB J., 1999, 13(suppl): S3-S15.Google Scholar
  6. 6.
    Huang, C. M., Ye, H., Xu, J. H. et al., Effects of extremely low frequency weak magnetic fields on the intracellular free calcium concentration in PC-12 tumor cells, J. Biomed. Eng., 2000, 79(1): 63–65.Google Scholar
  7. 7.
    Allen, F. D., Hung, C. T., Pollack, S. R. et al., Comparison of the [Ca2+]i response to fluid of MC3T3-E1, ROS17/2.8 and cultured primary osteoblast-like cells, Cell Eng., 1996, 1(1): 117–124.Google Scholar
  8. 8.
    Brighton, C. T., Wang, W., Seldes, R. et al., Signal transduction in electrically stimulated bone cells, J. Bone Joint Surg, 2001, 83-A(10): 1514.Google Scholar
  9. 9.
    McAlloster, T. N., Frangos, J. A., Steady and transient fluid shear stress stimulate NO release in osteoblasts through distinct biochemical pathways, J. Bone Miner Res., 1999, 14(6): 930–936.CrossRefGoogle Scholar
  10. 10.
    Zou, S. B., Chen, L. Y., Kang, H. G., Intracellular calcium signaling system, Chemistry of life, 2000, 20(6): 254–256.Google Scholar
  11. 11.
    Hung, C. T., Allen, F. D., Pollack, S. R. et al., Intracellular Ca2+ stores and extracellular Ca2+ are required in the real-time Ca2+ response of bone cells experiencing fluid flow, J. Biomech., 1996, 29(11): 1411–1417.CrossRefGoogle Scholar
  12. 12.
    Chen, N. X., Ryder, K. D., Pavalko, F. M. et al., Ca2+ regulates fluid shear-induced cytoskeletal reorganization and expression in osteoblasts, Am. J. Physiol., 2000, 278(5): C989–997.Google Scholar
  13. 13.
    Robey, P. G., Temine, J. D., Human bone cellsin vitro, Calcified Tissue Int., 1985, 37: 453–460.CrossRefGoogle Scholar
  14. 14.
    Zhang, X. Z., Kuang, Z. B., Cai, S. X. et al., The development of a cyclic four-point-bend loading device with uniaxial strain to cells, Medical Equipment Journal, 1999, 83(4): 6–8.Google Scholar
  15. 15.
    Wang, H. B., Lu, X., Wang, Y. L. L. et al., The effects of the mechanical strain on the adhesion and projective area and visco-elasticity of osteoblast, Acta Biophysica Sinica, 2001, 28(1): 103–107.Google Scholar
  16. 16.
    Peake, M. A., Cooling, L. M., Magnay, J. L. et al., Selected contribution: regulatory pathways involved in mechanical induction of c-fos gene expression in bone cells, J. Appl. Physiol., 2000, 89(6): 2498–2507.Google Scholar
  17. 17.
    Langille, B. L., Morphologic responses of endothelium to shear stress: reorganization of the adherent junction, Microcirculation, 2001, 8(3): 195–206.Google Scholar
  18. 18.
    Haier, J., Nicolson, G. L., PTEN regulates tumor cell adhesion of colon carcinoma cells under dynamic conditions of fluid flow, Oncogene, 2002, 21(9): 1450–1460.CrossRefGoogle Scholar
  19. 19.
    Buckley, K. A., Wagstaff, S. C., McKay, G. et al., Parathyroid hormone potentiates nucleotide-induced [Ca2+]i release in rat osteoblasts independently of Gq activation or cyclic monophosphate accumulation: A mechanism for localizing systemic responses in bone, J. Biol. Chem., 2001, 276(12): 9565–9571.CrossRefGoogle Scholar
  20. 20.
    Green, J., Schotland, S., Stauber, D. J. et al., Cell-matrix interaction in bone: type I collagen modulates signal transduction in osteoblast-like cells, Am. J. Physiol., 1995, 268(Sptl): c1090-c1103.Google Scholar
  21. 21.
    Guan, Y. Y., Kwan, C. Y., He, H. et al., Effects of Panal motoginseng saponins on receptor-operated Ca2+ channels in vascular smooth muscle, Acta Pharm. Sinica, 1994, 15(5): 392–398.Google Scholar
  22. 22.
    Mikuni-Takagaki, Y., Mechanical responses and signal transduction pathways in stretched osteocytes, J. Bone Miner Metab., 1999, 17(1): 57–60.CrossRefGoogle Scholar

Copyright information

© Science in China Press 2003

Authors and Affiliations

  • Liling Tang
    • 1
  • Yuanliang Wang
    • 1
  • Li Gu
    • 1
  • Shaoxi Cai
    • 1
  • Aihua Su
    • 1
  1. 1.Bio-engineering CollegeChongqing UniversityChongqingChina

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