Chinese Science Bulletin

, Volume 45, Issue 14, pp 1266–1270 | Cite as

Induction of matrix metalloproteinase-9 and -2 activity in mouse blastocyst by fibronectin-integrin interaction

  • Guodong Tie
  • Yujing Cao
  • Xingxu Zhao
  • Enkui Duan


Fibronectin, a major extracellular matrix, plays an important role in embryo implantation by mediating embryo adhesion and outgrowth. In this work, mouse blastocysts produced pro-matrix metalloproteinase-9, pro-matrix metalloproteinase-2 and 64 ku matrix metalloproteinase-2 when they were co-cultured with fibronectin. In contrast, mouse blastocysts did not produce these proteinases without fibronectin. Focal adhesion kinase is a fundamental molecule of integrin signaling pathway and its antisense oligodeoxynucleiotide inhibited blastocyst matrix metalloproteinases expression induced by fibronectin. The results indicated that fibronectin triggered matrix metalloproteinase-9 and -2 expression in mouse blastocyst through its integrin receptors and subsequent signaling pathway, which enhanced the synchronization of blastocyst invasiveness and uterine receptivity and ensured the accuracy of events relative to implantation in timing and spatiality.


fibronectin focal adhesion kinase (pp125FAKblastocyst matrix metalloproteinase-9 matrix metalloproteinase-2 


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Copyright information

© Science in China Press 2000

Authors and Affiliations

  • Guodong Tie
    • 1
    • 2
  • Yujing Cao
    • 1
  • Xingxu Zhao
    • 2
  • Enkui Duan
    • 1
  1. 1.State Key Laboratory of Reproductive Biology, Institute of ZoologyChinese Academy of SciencesBeijingChina
  2. 2.Department of Veterinary MedicineGansu Agricultural UniversityLanzhouChina

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