Chinese Science Bulletin

, Volume 49, Issue 3, pp 263–267 | Cite as

Ovicidal activity of two fungal pathogens (Hyphomycetes) againstTetranychus cinnabarinus (Acarina: Tetranichidae)



The carmine spider mite,Tetranychus cinnabarinus, is an economically important pest that devastates varieties of crops worldwide and develops significant resistance to common chemical pesticides, most of which lack ovicidal activity. In the present study, two isolates of entomopathogenic fungi,Beuaveria bassiana SG8702 andPaecilomyces fumosoroseus Pfr153, were bioassayed againstT. cinnabarinus eggs at 25 °C under a photophase of 12:12 (L:D). Infected eggs onVicia faba var.minor leaves failed to hatch due to distortion and shrinkage and had fungal outgrowths when maintained under moist conditions. Sprays ofB. bassiana conidia toT. cinnabarinus eggs (on leaves) at the concentrations of 58, 298 and 1306 conidia/mm2 (3 replicates per concentration and 35–65 fresh mite eggs per replicate) resulted in corrected egg mortalities of 20.4±4.2%, 36.0±7.6% and 64.6±12.5% (F=43.14,P <0.01), respectively; sprays ofP. fumosoroseus at 129, 402 and 2328 conidia/mm2 caused egg mortalities of 16.1±11.1%, 44.2±13.3%, and 63.4±11.7% (F= 15.37, P=0.01), respectively. In contrast, natural egg mortalities in blank controls were 7.8% and 10.3% only. Based on probit analysis, the estimates of LC50 with 95% confidential limits were 548 (393–858) conidia/mm2 forB. bassiana and 914 (625–1550) forP. fumosoroseus, respectively. Apparently, both fungal species were capable of infectingT. cinnabarinus eggs, but the ovicidal activity of theB. bassiana isolate was greater than that of theP. fumosoroseus isolate. This represents the first report on the ovicidal activity of the two entomopathogenic Hyphomycetes againstT. cinnabarinus.


Beauveria bassiana Paecilomycetes fumosoroseus Tetranychus cinnabarinus mite egg infection ovicidal activity entomopathogenic fungi bioassays 


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  1. 1.
    Hazan, A., Gerson, U., Tahori, A. S., Spider mite webbing, Part I: The production of webbing under various environmental conditions, Acarologia, 1974, 16: 68–84.Google Scholar
  2. 2.
    Hazan, A., Gerson, U., Tahori, A. S., Life history and life table of the carmine spider mite, Acarologia, 1973, 15: 414–440.Google Scholar
  3. 3.
    Bhagat, K. C., Singh, W., Some observations on the biology & behaviour of Carmine spider miteTetranychus cinnabarinus (Boisduval) (Acarina: Terranychitae)—A pest of brinjal vegetable, J. Adv. Zool., 1999, 20: 28–31.Google Scholar
  4. 4.
    Gerson, U., Cohen, E., Resurgences of spider mites (Acari: Tetranychidae) induced by synthetic pyrethroids, Exp. Appl. Acarol., 1989, 6: 29–46.CrossRefGoogle Scholar
  5. 5.
    Yu, Y. J., Zhejiang standards for production of safe vegetables and control of pesticide application, Integrated Pest Management in Relation to Safe Agricultural Products (eds. Yu, Y. J., Zhang, Q. H.), Beijing: China Agricultural Press, 11–16.Google Scholar
  6. 6.
    Dagli, F., Tunc, I., Dicofol resistance inTetranychus cinnabarinus: Resistance and stability of resistance in populations from Antalya, Turkey, Pest Manag. Sci., 2001, 57: 609–614.CrossRefGoogle Scholar
  7. 7.
    James, D. G., Price, T. S., Imidacloprid boosts TSSM egg production, Agrichem. Environ. News, 2002, 189: 1–11.Google Scholar
  8. 8.
    Guo, F. Y., Zhang, Z. Q., Zhao, Z. M., Pesticide resistance ofTetranychus cinnabarinus (Acari: Tetranychidae) in China: A review, Syst. Appl. Acarol., 1998, 3: 3–7.Google Scholar
  9. 9.
    Kazak, C., Karut, K., Kasap, I. et al., The potential of the Hatay population ofPhytoseiulus persimilis to control the carmine spider miteTetranychus cinnabarinus in strawberry in Silifke Icel, Turkey, Phytoparasitica, 2002, 30: 451–458.CrossRefGoogle Scholar
  10. 10.
    Sugasawa, J., Kitashima, Y., Gotoh, T., Hybrid affinities between the green and red forms of the two-spotted spider miteTetranychus urticae (Acari: Tetranychidae) under laboratory and semi-natural conditions, Appl. Entomol. Zool., 2002, 37: 127–139.CrossRefGoogle Scholar
  11. 11.
    Tadmor, Y., Lewinsohn, E., Abo-Moch, F. et al., Antibiosis of maize inbred lines to the carmine spider mite,Tetranychus cinnabarinus, Pytoparasitica, 1999, 27: 35–41.CrossRefGoogle Scholar
  12. 12.
    Mansour, F., Shain, Z., Karchi, Z. et al., Resistance of selected melon lines to the carmine spider miteTetranychus cinnabarinus (Acari: Tetranychidae)-field and laboratory experiments, Bull. Entomol. Res., 1994, 84: 265–267.CrossRefGoogle Scholar
  13. 13.
    Chandler, D., Davidson, G., Pell, J. K. et al., Fungal biocontrol of Acari, Biocontrol. Sci. Technol., 2000: 357–384.Google Scholar
  14. 14.
    van der Geest, L. P. S., Elliot, S. L., Breeuwer, J. A. J. et al., Diseases of mites, Exp. Appl. Acrol., 2000, 24: 497–560.CrossRefGoogle Scholar
  15. 15.
    Sztejnberg, A., Doron-Shloush, S., Gerson, U., The biology of the acaropathogenic fungusHirsutella kirchneri, Biocontrol Sci. Technol., 1997, 7: 577–590.CrossRefGoogle Scholar
  16. 16.
    Batta, Y. A., Production and testing of novel formulations of the entomopathogenic fungusMetarhizium anisopliae (Metschinkoff) Sorokin (Deuteromycotina: Hyphomycetes), Crop Prot., 2003, 22: 415–422.CrossRefGoogle Scholar
  17. 17.
    Feng, M. G., Johnson, J. B., Kish, L. P., Survey of entomopathogenic fungi naturally infecting cereal aphids (Homoptera: Aphididae) of irrigated grain crops in southwestern Idaho, Environ. Entomol., 1990, 19: 1534–1542.Google Scholar
  18. 18.
    Feng, M. G., Poprawski, T. J., Khachatourians, G. G., Production, formulation and application of the entomopathogenic fungusBeauveria bassiana for insect control: Current status, Biocontrol Sci. Technol., 1994, 4: 3–34.CrossRefGoogle Scholar
  19. 19.
    Tang, Q. Y., Feng, M. G., DPS Data Processing System for Practical Analysis, Beijing: Science Press, 2002, 1–648.Google Scholar
  20. 20.
    Lacey, L. A., Kirk, A. A., Millar, L. et al., Ovicidal and larvicidal activity of conidia and blastospores ofPaecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) againstBemisia argentifolii (Homoptera: Aleyrodidae) with a description of a bioassay system allowing prolonged survival of control insects, Biocontrol Sci. Technol., 1999, 9: 9–18.CrossRefGoogle Scholar
  21. 21.
    Samuels, R. L., Coracini, D. L. A., Martins dos Santos, C. A. et al., Infection ofBlissus antillus (Hemiptera: Lygaeidae) eggs by the entomopathogenic fungiMetarhizium anisopliae andBeauveria bassiana, Biol. Control, 2002, 23: 269–273.CrossRefGoogle Scholar
  22. 22.
    Feng, M. G., Liu, C. L., Xu, J. H. et al., Modeling and biological implication of the time-dose-mortality data for the entomophthoralean fungus,Zoophthora anhuiensis on the green peach aphid,Myzus percisae, J. Invertebr. Pathol., 1998, 72: 246–251.CrossRefGoogle Scholar
  23. 23.
    Feng, M. G., Poprawski, T. J., Robustness for the processing of bioassay data of microbial control agents based on time-dose-mortality model, Subtrop. Plant Sci., 1999, 51: 36–38.Google Scholar

Copyright information

© Science in China Press 2004

Authors and Affiliations

  1. 1.Institute of Applied Entomology, College of Agricultural Sciences and BiotechnologyZhejiang UniversityHangzhouChina
  2. 2.Institute of Microbiology, College of Life SciencesZhejiang UniversityHangzhouChina

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