Ovicidal activity of two fungal pathogens (Hyphomycetes) againstTetranychus cinnabarinus (Acarina: Tetranichidae)
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The carmine spider mite,Tetranychus cinnabarinus, is an economically important pest that devastates varieties of crops worldwide and develops significant resistance to common chemical pesticides, most of which lack ovicidal activity. In the present study, two isolates of entomopathogenic fungi,Beuaveria bassiana SG8702 andPaecilomyces fumosoroseus Pfr153, were bioassayed againstT. cinnabarinus eggs at 25 °C under a photophase of 12:12 (L:D). Infected eggs onVicia faba var.minor leaves failed to hatch due to distortion and shrinkage and had fungal outgrowths when maintained under moist conditions. Sprays ofB. bassiana conidia toT. cinnabarinus eggs (on leaves) at the concentrations of 58, 298 and 1306 conidia/mm2 (3 replicates per concentration and 35–65 fresh mite eggs per replicate) resulted in corrected egg mortalities of 20.4±4.2%, 36.0±7.6% and 64.6±12.5% (F=43.14,P <0.01), respectively; sprays ofP. fumosoroseus at 129, 402 and 2328 conidia/mm2 caused egg mortalities of 16.1±11.1%, 44.2±13.3%, and 63.4±11.7% (F= 15.37, P=0.01), respectively. In contrast, natural egg mortalities in blank controls were 7.8% and 10.3% only. Based on probit analysis, the estimates of LC50 with 95% confidential limits were 548 (393–858) conidia/mm2 forB. bassiana and 914 (625–1550) forP. fumosoroseus, respectively. Apparently, both fungal species were capable of infectingT. cinnabarinus eggs, but the ovicidal activity of theB. bassiana isolate was greater than that of theP. fumosoroseus isolate. This represents the first report on the ovicidal activity of the two entomopathogenic Hyphomycetes againstT. cinnabarinus.
KeywordsBeauveria bassiana Paecilomycetes fumosoroseus Tetranychus cinnabarinus mite egg infection ovicidal activity entomopathogenic fungi bioassays
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