Cloning of theAspergillus awamori glucoamylase gene and expression inSaccharomyces cerevisiae
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TheA. awamori glucoamylase I gene was obtained by RT-PCR and inserted into the plasmid pAC1. We constructed an expression vector pAC1-CA, which was used to transform the yeast S.cerevisiae AS 2.1364 without auxotrophic marker. The transformant secreted glucoamylase into the medium efficiently and degraded starch. The glucoamylase activity of the culture filtrate is up to 8.4 U/mL.
KeywordsAspergillus awamori glucoamylase gene cloning and expression Saccharomyces cerevisiae
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