Chinese Science Bulletin

, Volume 43, Issue 10, pp 855–859 | Cite as

Cloning of theAspergillus awamori glucoamylase gene and expression inSaccharomyces cerevisiae

  • Wang Yongji 
  • Liu Hongdi 
  • Sun Tong 
  • Zhang Shuzheng 


TheA. awamori glucoamylase I gene was obtained by RT-PCR and inserted into the plasmid pAC1. We constructed an expression vector pAC1-CA, which was used to transform the yeast S.cerevisiae AS 2.1364 without auxotrophic marker. The transformant secreted glucoamylase into the medium efficiently and degraded starch. The glucoamylase activity of the culture filtrate is up to 8.4 U/mL.


Aspergillus awamori glucoamylase gene cloning and expression Saccharomyces cerevisiae 


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Copyright information

© Science in China Press 1998

Authors and Affiliations

  • Wang Yongji 
    • 1
  • Liu Hongdi 
    • 1
  • Sun Tong 
    • 1
  • Zhang Shuzheng 
    • 1
  1. 1.Institute of MicrobiologyChinese Academy of SciencesBeijingChina

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