Science in China Series C: Life Sciences

, Volume 46, Issue 1, pp 58–66 | Cite as

Analysis of nucleolar pre-rRNA processing sites in pea (Pisum sativum)

  • Hong Long
  • Xianlu Zeng
  • Mingda Jiao
  • Bo Hu
  • Haijing Sun
  • Zhenlan Liu
  • Liyong Zhang
  • Shui Hao


The location of rRNA processing was analyzed by usingin situ hybridization with ITS1 probe and immunolabeling of anti-fibrillarin mAb in pea (Pisum sativum) root pole cells. The results showed that rRNA processing sites were in dense fibrillar components (DFCs) and granular components (GCs), but not in fibrillar centers (FCs). Low doses of actinomycin D (AMD) treatment can selectively suppress pre-rRNA synthesis but cannot disturb the processing of preformed pre-rRNAs. With AMD treatment prolonged, the density of labeled signals gradually decreased, indicating the preformed pre-rRNAs were gradually processed.


Pisum sativum nucleolus rRNA processing fibrillar centres dense fibrillar components granular components 


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Copyright information

© Science in China Press 2003

Authors and Affiliations

  • Hong Long
    • 1
    • 2
  • Xianlu Zeng
    • 1
  • Mingda Jiao
    • 1
  • Bo Hu
    • 1
  • Haijing Sun
    • 1
  • Zhenlan Liu
    • 1
  • Liyong Zhang
    • 1
    • 3
  • Shui Hao
    • 1
  1. 1.Institute of Genetics and CytologyNortheast Normal UniversityChangchunChina
  2. 2.College of Life ScienceNankai UniversityTianjinChina
  3. 3.National Laboratory of Molecular Oncology, Cancer InstituteChinese Academy of Medical Sciences & Peking Union Medical CollegeBeijingChina

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