Advertisement

Science in China Series C: Life Sciences

, Volume 46, Issue 1, pp 58–66 | Cite as

Analysis of nucleolar pre-rRNA processing sites in pea (Pisum sativum)

  • Hong Long
  • Xianlu Zeng
  • Mingda Jiao
  • Bo Hu
  • Haijing Sun
  • Zhenlan Liu
  • Liyong Zhang
  • Shui Hao
Article

Abstract

The location of rRNA processing was analyzed by usingin situ hybridization with ITS1 probe and immunolabeling of anti-fibrillarin mAb in pea (Pisum sativum) root pole cells. The results showed that rRNA processing sites were in dense fibrillar components (DFCs) and granular components (GCs), but not in fibrillar centers (FCs). Low doses of actinomycin D (AMD) treatment can selectively suppress pre-rRNA synthesis but cannot disturb the processing of preformed pre-rRNAs. With AMD treatment prolonged, the density of labeled signals gradually decreased, indicating the preformed pre-rRNAs were gradually processed.

Keywords

Pisum sativum nucleolus rRNA processing fibrillar centres dense fibrillar components granular components 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Shaw, P. J., Jordan, G. E., The nucleolus, Annu. Rev. Cell Dev Biol., 1995, 11: 93–121.CrossRefPubMedGoogle Scholar
  2. 2.
    Hao, S., Research advances on the ultrastructure and function of nucleolus, in Trends in Cell Biology (eds. Zheng, G. C., Zhai, Z. H.) (in Chinese), Vol. 3, Beijing: Beijing Normal University Press, 1995, 177–186.Google Scholar
  3. 3.
    Eichler, D. C., Craig, N. C., Processing of eukaryotic ribosomal RNA, Prog. Nucleic Acid Res. Mol. Biol., 1994, 49: 97–239.Google Scholar
  4. 4.
    Melese, T., Xue, Z., The nucleolus: An organelle formed by the act of building a ribosome, Curr. Opin. Cell Biol., 1995, 7: 319–324.CrossRefPubMedGoogle Scholar
  5. 5.
    Granboulan, N., Granboulan, P., Cytochimie ultrastructurale du nucleole, Exp. Cell Res., 1965, 38: 604–619.CrossRefPubMedGoogle Scholar
  6. 6.
    Goessens, G., High resolution autoradiographic studies of Ehrlich tumor cell nuclei, Exp. Cell Res., 1976, 100: 88–94.CrossRefPubMedGoogle Scholar
  7. 7.
    Goessens, G., Nucleolar structure, Int. Rev. Cytol., 1984, 87: 107–158.CrossRefPubMedGoogle Scholar
  8. 8.
    Fakan, S., High resolution autoradiographic studies on chromatin functions, Cell Nucleus, 1978, 5: 3–53.Google Scholar
  9. 9.
    Puvion, E., Moyne, G.,In situ localization of RNA structures, Cell Nucleus, 1981, 8: 59–115.Google Scholar
  10. 10.
    Fischer, D., Weisenberger, D., Scheer, U., Assigning functions to nucleolar structures, Chromosoma, 1991, 101: 133–140.CrossRefPubMedGoogle Scholar
  11. 11.
    Beven, A. F., Lee, R., Razaz, M. et al., The organization of ribosomal RNA processing correlates with the distribution of nucleolar snRNAs, J. Cell Sci., 1996, 109: 1241–1251.PubMedGoogle Scholar
  12. 12.
    Puvion-Dutilleul, F., Bachellerie, J. P., Puvion, E., Nucleolar organization of HeLa cells as studied byin situ hybridization, Chromosoma, 1991, 100: 395–409.CrossRefPubMedGoogle Scholar
  13. 13.
    Antonio Cerdido, Fransisco Javier Medina, Subnucleolar location of fibrillarin and variation in its levels during the cell cycle and during differentiation of plant cells, Chromosoma, 1995, 103: 625–634.CrossRefPubMedGoogle Scholar
  14. 14.
    Lazdins, I. B., Delannoy, M., Sollner-Webb, B., Analysis of nucleolar transcription and processing domains and pre-rRNA movements byin situ hybridization, Chromosoma, 1997, 105: 481–495.CrossRefPubMedGoogle Scholar
  15. 15.
    Puvion-Dutilleul, F., Mazan, S., Nicoloso, M. et al., Localization of U3 RNA molecules in nucleoli of HeLa and mouse 3T3 cells by high resolution in situ hybridization, Eur. J. Cell Biol., 1991, 56: 149–162.Google Scholar
  16. 16.
    Perry, R. P., Kelley, D. E., Inhibition of RNA synthesis by actinomycin D: Characteristic dose-response of different RNA species, J. Cell Physiol., 1970, 76: 127–139.CrossRefPubMedGoogle Scholar
  17. 17.
    Fetheston, J., Werner, E., Patterson, R., Processing of the external transcribed spacer of murine rRNA and site of actinomycinD, Nucleic Acids Res., 1984, 12: 7187–7198.CrossRefGoogle Scholar
  18. 18.
    Hu, B., Xing, M., The puff-like structure: an intranuclear structure for synthesis of ribonucleic acid polymerase II transcripts as revealed by 5-bromouridine-5′-triphosphate labelling and immunoelectron microscopy, Acta Bot. Sin. (in Chinese), 1998, 40: 395–400.Google Scholar
  19. 19.
    Geuskens, M., Bernhard, W., Cytochimie ultrastructurale dunucléole, Exp. Cell Res., 1966, 44: 579–598.CrossRefPubMedGoogle Scholar
  20. 20.
    Royal, A., Simard, R., RNA synthesis in the ultrastructural and biochemical components of the nucleolus of Chinese hamster ovary cells, J. Cell Biol., 1975, 66: 577–585.CrossRefPubMedGoogle Scholar
  21. 21.
    Stahl, A., Wachtler, F., Hartung, M. et al., Nucleoli, nucleolar chromosomes and ribosomal gene in the human spermatocyte, Chromosoma, 1991, 101: 231–244.CrossRefPubMedGoogle Scholar
  22. 22.
    Tao, W., He, M. -Y., Hao, S., Ultrastructural localization and analysis of active gene transcription in nucleolus of onion (Allium cepa), Chinese Science Bulletin, 2000, 45(22): 2403–2408.Google Scholar
  23. 23.
    Susan, A. G., Anton, B., U3 snoRNA may recycle through different compartments of the nucleolus, Chromosoma, 1997, 105: 401–406.CrossRefGoogle Scholar
  24. 24.
    Gerbi, S. A., Savino, R., Stebbins-Boaz, B. et al., A role for U3 small nuclear ribonucleoprotein in the nucleolus? in The Ribosome-Structure, Function and Evolution (eds. Hill, W. E., Dahlberg, A., Garrett, R. A. et al.), Washington DC: American Society for Microbiology, 1990, 452–469.Google Scholar
  25. 25.
    Thiry, M., Thiry-Blaise, L., Locating transcribed and non-transcribed rDNA spacer sequences within the nucleolus byin situ hybridization and immunoelectron microscopy, Nuclei Acids Res., 1991, 19: 11–15.CrossRefGoogle Scholar
  26. 26.
    Puvion-Dutilleul, F., Mazan, S., Nicoloso, M. et al., Alterations of nucleolar ultrastructure and ribosome biogenesis by actinomycin D. Implications for U3 snRNP function, Eur. J. Cell Biol., 1992, 58: 149–162.PubMedGoogle Scholar
  27. 27.
    Shaw, P. J., Highett, M. I., Beven, A. F. et al., The nucleolar architecture of polymerase I transcription and processing, EMBO J., 1995, 14: 2896–2906.PubMedGoogle Scholar
  28. 28.
    Antonio Cerdido, Fransisco Javier Medina, Subnucleolar location of fibrillarin and variation in its levels during the cell cycle and during differentiation of plant cells, Chromosoma, 1995, 103: 625–634.CrossRefPubMedGoogle Scholar
  29. 29.
    Girard, J. P., Lehtonen, H., Caizergues-Ferrer, M. et al., GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast, EMBO J., 1992, 11: 673–682.PubMedGoogle Scholar
  30. 30.
    Tyc, K., Steitz, J. A., U3, U8 and U13 comprise a new class of mammalian snRNA localized in the cell nucleolus, EMBO J., 1989, 8: 3113–3119.PubMedGoogle Scholar
  31. 31.
    Baserga, S. J., Yang, X. D. W., Steitz, J. A., An intact box C sequence in the U3 snoRNA is required for binding of fibrillarin, the protein common to the major family of nucleolar snRNPs, EMBO J., 1991, 9: 2645–2651.Google Scholar
  32. 32.
    Schimmang, T., Tollervey, D., Kern, H. et al., A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability, EMBO J., 1989, 8: 4015–4024.PubMedGoogle Scholar
  33. 33.
    Tollervey, D., Lehtonen, H., Carmo-Fonseca, M. et al., The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast, EMBO J., 1991, 10: 573–583.PubMedGoogle Scholar
  34. 34.
    Tollervey, D., Lehtonen, H., Jansen, R. et al., Temperature-sensitive mutations demonstrate roles for yeast fibrillarin in pre-rRNA processing, pre-rRNA methylation, and ribosome assembly, Cell, 1993, 72: 443–457.CrossRefPubMedGoogle Scholar
  35. 35.
    Ochs, R. L., Lischwe, M. A., Spohn, W. H. et al., Fibrillarin: a new protein of the nucleolus identified by autoimmune sera, Biol. Cell, 1985, 54: 123–134.PubMedGoogle Scholar
  36. 36.
    Puvion-Dutilleul, F., Mazan, S., Nicoloso, M. et al., Localization of U3 RNA molecules in nucleoli of HeLa and mouse 3T3 cells by high resolutionin situ hybridization, Eur. J. Cell Biol., 1991, 56: 149–162.Google Scholar
  37. 37.
    Testillano, P. S., Sanchez-Pina, M. A., Lopez-lglesias, C. et al., Distribution of B-36 nucleolar protein in relation to transcriptional activity in plant cells, Chromosoma, 1992, 102: 41–49.CrossRefGoogle Scholar

Copyright information

© Science in China Press 2003

Authors and Affiliations

  • Hong Long
    • 1
    • 2
  • Xianlu Zeng
    • 1
  • Mingda Jiao
    • 1
  • Bo Hu
    • 1
  • Haijing Sun
    • 1
  • Zhenlan Liu
    • 1
  • Liyong Zhang
    • 1
    • 3
  • Shui Hao
    • 1
  1. 1.Institute of Genetics and CytologyNortheast Normal UniversityChangchunChina
  2. 2.College of Life ScienceNankai UniversityTianjinChina
  3. 3.National Laboratory of Molecular Oncology, Cancer InstituteChinese Academy of Medical Sciences & Peking Union Medical CollegeBeijingChina

Personalised recommendations