Comparison of vitamin E, L-carnitine and melatonin in ameliorating carbon tetrachloride and diabetes induced hepatic oxidative stress
- 154 Downloads
This study aimed to investigate whether treatments with vitamin E, L-carnitine and melatonin can protect against CCl4 and diabetes-induced hepatic oxidative stress. Hepatic oxidative stress was performed in rats through 50% v/v carbon tetrachloride (CCl4) (1 ml/kg/3days, i.p.), and through diabetes mellitus induced by streptozotocin (STZ) (40 mg/kg, i.p.). Vitamin E (100 mg/kg/day, i.p), L-carnitine (300 mg/kg/day, i.p.) and melatonin (10 mg/kg/day, i.p.) were injected for a period of 6 weeks. Thereafter, changes in serum glucose level, liver function tests, hepatic malondialdehyde (MDA) content, hepatic reduced glutathione (GSH) content, hepatic superoxide dismutase (SOD) activity, and serum total antioxidant capacity (TAC) level were evaluated. In CCl4-induced liver fibrosis, the efficacy order was melatonin > L-carnitine > vitamin E, while in STZ-induced diabetes, the efficacy order was vitamin E ≥ melatonin > L-carnitine. In conclusion, these data indicate that low dose of melatonin is more effective than high doses of vitamin E and L-carnitine in reducing hepatic oxidative stress induced by CCl4 and diabetes. Moreover, the potent effect of vitamin E in ameliorating diabetes can be linked not only to the antioxidant actions, but also to the superior effect in reducing diabetes-induced hyperglycaemia. Meanwhile, potency of L-carnitine was nearly the same in CCl4 and diabetes-induced liver damage.
Key wordsVitamin E L-carnitine Melatonin Carbon tetrachloride Diabetes
Unable to display preview. Download preview PDF.
- 3.Boll, M., Weber, L.W., Becker, E., Stampfl, A. (2001): Mechanism of carbon tetrachloride-induced hepatotoxicity. Hepatocellular damage by reactive carbon tetrachloride metabolites.Z Naturforsch,56, 649–659.Google Scholar
- 7.Chawla, R.K., Lewis, F.W., Kutner, M.H., Bate, D.M., Roy, R.G.B., Rudman, D. (1984): Plasma cysteine, cystine, and glutathione in cirrhosis.Gastroenterol,87: 770–775.Google Scholar
- 22.Oliveira, H.R., Curi, R., Carpinelli, A.R. (1999): Glucose induces an acute increase of superoxide dismutase activity in incubated rat pancreatic islets.Am J Physiol,276, 507–510.Google Scholar
- 25.Rajasekar, P., Kaviarasan, S., Anuradha, C.V. (2005): L-carnitine administration prevents oxidative stress in high fructose-fed insulin resistant rats.Diabetol Croat,34: 21–28.Google Scholar
- 31.Trinder, P. (1969): Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor.Ann Clin Biochem,6, 24–28.Google Scholar
- 33.Vozarova, B., Stefan, N., Lindsay, R.S., Saremi, A., Pratley, R.E., Bogardus, C., Tataranni, P.A. (2002): High alanine aminotransferase is associated with decrease hepatic insulin sensitivity and predicts the development of type 2 diabetes.Diabetes,51, 1189–1195.Google Scholar