Advertisement

Annals of Microbiology

, Volume 58, Issue 1, pp 169–171 | Cite as

Conformational study of protein in some species ofFusarium by FT-IR spectroscopy

  • Jiarong Wan
  • Yi Liu
  • Lixin Lan
  • Yan Gao
  • Ming Nie
  • Juan Zhou
Methods Research Note

Abstract

A novel method of estimating conformation of protein secondary structure withinFusarium cells under the influence of growth cultures and bodies was studies by Fourier-Transform Infrared (FT-IR) spectroscopy combined with a deconvolution procedure of amide I band. After infrared data collection and curve-fitting process, the percentages of secondary structure components (a-helix, β-sheet, turn, random coil and β-antiparallel) were obtained. It showed structural diversity among differentFusarium species and variations caused by growth cultures and bodies. The method provides a rapidly quantitative technique to study cell structure of fungi.

Key words

Fusarium FT-IR spectroscopy protein secondary structure amide I band 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ahmed A., Tajimir-Riahi H.A., Carpentier R. (1995). A quantitative secondary structure analysis of the 33 kDa extrinsic polypeptide of photosystem II by FTIR spectroscopy. FEBS Lett., 363: 65–68.CrossRefPubMedGoogle Scholar
  2. Ellepola S.W., Choi S.M., Ma C.Y. (2005). Conformational study of globulin from rice (Oryza sativa) seeds by Fourier-transform infrared spectroscopy. Int. J. Biol. Macromol., 37: 12–20.CrossRefPubMedGoogle Scholar
  3. Jurgenson J.E., Bowden R.L., Zeller K.A., Leslie J.F., Alexander N.J., Plattner R.D. (2002). A genetic map ofGibberella zeae (Fusarium graminearum). Genetics, 160: 1451–1460.PubMedGoogle Scholar
  4. Logrieco A., Moretti A., Ritieni A., Chelkowski J., Altomare C., Bottalico A., Randazzo G. (1993). Natural occurrence of beauvericin in perharvestFusarium subglutinans infected corn ears in Poland. J. Agric. Food Chem., 41: 2149–2152.CrossRefGoogle Scholar
  5. Lutz M.P., Feichtinger G., Défago G., Duffy B. (2003). MycotoxigenicFusarium and deoxynivalenol production repress chitinase gene expression in the biocontrol agentTrichoderma atroviride P1. Appl. Environ. Microbiol., 69: 3077–3084.CrossRefPubMedGoogle Scholar
  6. Mohašek-Grošev V., Božac R., Puppels G.J. (2001). Vibrational spectroscopic characterization of wild growing mushrooms and toadstools. Spectrochim. Acta A, 57: 2815–2829.CrossRefGoogle Scholar
  7. Nelson P.E., Toussoun T.A., Marasas W.F.O. (1983).Fusarium Species: An Illustrated Manual for Identification, The Pennsylvania State University Press, University Park, Pennsylvania.Google Scholar
  8. Oberg K.A., Ruysschaert J.M., Goormaghtigh E. (2004). The optimization of protein secodary structure determination with infrared and circular dichroism spectra. Eur. J. Biochem., 271: 2937–2948.CrossRefPubMedGoogle Scholar
  9. Paris C., Lecomte S., Coupry C. (2005). ATR-FTIR spectroscopy as a way to identify natural protein-based materials, tortoiseshell and horn, from their protein-based imitation, galalith. Spectrochim. Acta A, 62: 532–538.CrossRefGoogle Scholar
  10. Subirade M., Kelly I., Guéguen J.G., Pézolet M. (1998). Molecular basis of film formation from a soybean protein: comparison between the conformation of glycinin in aqueous solution and in films. Int. J. Biol. Macromol., 23: 241–249.CrossRefPubMedGoogle Scholar
  11. Wang G.C., Zheng Z., Ye Q.M., Zhang C.L. (1996). Guide to Identification of CommonFusarium Species, China Agricultural Scientech Press, Beijing.Google Scholar

Copyright information

© University of Milan and Springer 2008

Authors and Affiliations

  • Jiarong Wan
    • 1
  • Yi Liu
    • 1
  • Lixin Lan
    • 1
  • Yan Gao
    • 1
  • Ming Nie
    • 2
  • Juan Zhou
    • 3
  1. 1.Hunan Chemical Industry Vocational InstituteHunanChina
  2. 2.Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity ScienceFudan UniversityShanghaiChina
  3. 3.Engineering, CollegeHunan Agricultural UniversityChangshaChina

Personalised recommendations