Conformational study of protein in some species ofFusarium by FT-IR spectroscopy
- 57 Downloads
A novel method of estimating conformation of protein secondary structure withinFusarium cells under the influence of growth cultures and bodies was studies by Fourier-Transform Infrared (FT-IR) spectroscopy combined with a deconvolution procedure of amide I band. After infrared data collection and curve-fitting process, the percentages of secondary structure components (a-helix, β-sheet, turn, random coil and β-antiparallel) were obtained. It showed structural diversity among differentFusarium species and variations caused by growth cultures and bodies. The method provides a rapidly quantitative technique to study cell structure of fungi.
Key wordsFusarium FT-IR spectroscopy protein secondary structure amide I band
Unable to display preview. Download preview PDF.
- Nelson P.E., Toussoun T.A., Marasas W.F.O. (1983).Fusarium Species: An Illustrated Manual for Identification, The Pennsylvania State University Press, University Park, Pennsylvania.Google Scholar
- Wang G.C., Zheng Z., Ye Q.M., Zhang C.L. (1996). Guide to Identification of CommonFusarium Species, China Agricultural Scientech Press, Beijing.Google Scholar