Annals of Microbiology

, Volume 58, Issue 1, pp 109–114 | Cite as

Production of actinomycin-D by a new isolate,Streptomyces sindenensis

  • Vandana Praveen
  • Chandra Kant Mani Tripathi
  • Vinod Bihari
  • Suresh Chandra Srivastava
Industrial Microbiology Original Articles


An actinomycete strain, designated as C-5, was isolated from the soil sample collected from steel plant effluent sediment. The strain produced actinomycin-D in substantial quantities (80 mg/L). The strain C-5 was taxonomically characterised on the basis of morphological and phenotypic characteristics, genotypic data and phylogenetic analysis. A nearly complete 16S rRNA sequence of the isolate was determined and found to have 100% identity withStreptomyces sindenensis. Streptomyces sindenensis is not reported earlier to produce actinomycin-D. Anticancer activities were determined against various human cancer cell lines. Production of actinomycin-D was scaled up in bench scale fermentors and optimised to a titre of 120 mg/L.

Key words

actinomycin-D Streptomyces sindenensis anticancer antibiotic fermentation 


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Copyright information

© University of Milan and Springer 2008

Authors and Affiliations

  • Vandana Praveen
    • 1
  • Chandra Kant Mani Tripathi
    • 1
  • Vinod Bihari
    • 1
  • Suresh Chandra Srivastava
    • 2
  1. 1.Fermentation Technology DivisionCentral Drug Research InstituteLucknow
  2. 2.Department of BotanyUniversity of LucknowLucknowIndia

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