Skip to main content
Log in

Factors affecting the coaggregation ability of vaginal Lactobacilli withCandida spp.

  • Applied Microbiology
  • Short Communication
  • Published:
Annals of Microbiology Aims and scope Submit manuscript

Abstract

In this study, the capacity of 30 strains of lactobacilli to coaggregate withCandida albicans ATCC 10239,C. albicans AJD 180 andCandida krusei ATCC 6258 were studiedin vitro. A marked coaggregation withC. albicans was observed for two strains ofLactobacillus crispatus, a strain ofLactobacillus cellobiosus and a strain ofLactobacillus salivarius. Coaggregation occurred at a pH range from 3 to 7, some strains showing optimal binding at high and other strains at low pH. Treatment of lactobacilli at 70 or 85°C for 20 min or treatment of the bacteria with pepsin abolished their capacity of coaggregation. The results may be of importance when trying to establish probiotics for vaginal use.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Aslim B., Kilic E. (2006). Some probiotic properties of vaginal Lactobacilli isolated from healthy women. Jpn. J. Infect. Dis., 59: 249–253.

    PubMed  Google Scholar 

  • Boris S., Suárez J.E., Vázquez F., Barbés C. (1998). Adherence of human vaginal lactobacilli to vaginal epithelial cells and interaction with uropathogens. Infect. Immun., 66: 1985–1989.

    CAS  PubMed  Google Scholar 

  • Chan R.C.Y., Reid G., Irvin R.T., Bruce A.W., Costerton J.W. (1995). Competitive exclusion of uropathogens from human uropathogens from human epithelial cells byLactobacillus whole cells and cell wall fragments. Infect. Immun., 47: 84–89.

    Google Scholar 

  • Grimaudo N.J., Nesbitt W.E. (1997). Coaggregation ofCandida albicans with oralFusobacterium species. Oral Microbiol Immunol., 12: 168–173.

    Article  CAS  PubMed  Google Scholar 

  • Jabra-Rizk M.A., Falker W.A., Merz W.G., Kelley J.I., Baqui A.A.M.A., Meiller T.F. (1999). Coaggregation ofCandida dubliniensis withFusobacterium nucleatum. J. Clin. Microbiol. 37: 1464–1468.

    CAS  PubMed  Google Scholar 

  • Kelstrup J., Funder-Nielsen T.D. (1974). Aggregation of oral streptococci withFusobacterium andActinomyces. J. Biol. Buccale, 2: 347–362.

    CAS  PubMed  Google Scholar 

  • Kilic E., Aslim B., Taner, Z. (2005). Susceptibility to some antifungal drugs of vaginal Lactobacilli isolated from healthy women. Drug Metabol. Drug Interact., 21: 67–74.

    CAS  PubMed  Google Scholar 

  • Kmet V., Callegari M.L., Bottazzi V., Morelli L. (1995). Aggregation promoting factor in pig intestinalLactobacillus strains. Lett. Appl. Microbiol., 21: 351–353.

    Article  CAS  PubMed  Google Scholar 

  • Kmet V., Lucchini F. (1997). Aggregation-promoting factor in human vaginal Lactobacillus strains. FEMS Immunol. Med. Microbiol., 19: 111–114.

    Article  CAS  PubMed  Google Scholar 

  • Mastromarino P., Brigidi P., Macchia S., Maggi L., Pirovano F., Trinchieri V., Conte U., Matteuzzi D. (2002). Characterization and selection of vaginalLactobacillus strains for the preparation of vaginal tablets. J. Appl. Microbiol., 93: 884–893.

    Article  CAS  PubMed  Google Scholar 

  • McGroarty J.A. (1993). Probiotic use of lactobacilli in the human female urogenital tract. FEMS Immunol. Med. Microbiol., 6: 251–264.

    Article  CAS  PubMed  Google Scholar 

  • Ocaňa V.S., Nader-Macías M.E. (2002). Vaginal lactobacilli: self- and coaggregation ability. Brit. J. Biomed. Sci., 59: 183–190.

    Google Scholar 

  • Reid G., McGroarty J.A., Angotti R., Cook R.L. (1988).Lactobacillus inhibitor production againstEscherichia coli and coaggregation ability with uropathogens. Can. J. Microbiol., 34: 344–351.

    Article  CAS  PubMed  Google Scholar 

  • Reid G., McGroarty J.A., Domingue P.A., Chow A.W., Bruce A.W., Eisen A., Costerton J.W. (1990). Coaggregation of urogenital bacteria in vitro andin vivo. Curr. Microbiol., 20: 47–52.

    Article  Google Scholar 

  • Reid G., Bruce A.W. (2003). Urogenital infections in women: Can probiotics help? Postgrad. Med. J., 79: 428–432.

    Article  CAS  PubMed  Google Scholar 

  • Schachtsiek M., Hammes P.W., Hertel C. (2004). Characterization ofLactobacillus coryniformis DSM 2001T surface protein Cpf mediating coaggregation with and aggregation among pathogens. Appl. Environ. Microbiol., 70: 7078–7085.

    Article  CAS  PubMed  Google Scholar 

  • Sobel J.D. (1996). Vaginits and vaginal flora: controversies about. Curr. Opin. Infect. Dis., 9: 42–47.

    Article  Google Scholar 

  • Steele C., Paul L., Fidel J. (2002). Cytokine and chemokine production by human oral and vaginal epithelial cells in response toCandida albicans. Infect. Immun., 70: 577–583.

    Article  CAS  PubMed  Google Scholar 

  • Sweet S., Wallace T., Samaranayake L. (1987). Determination of the cell surface hydrophobicity of oral bacteria using a modified hydrocarbon adherence method. FEMS Microbiol. Lett., 48: 159–163.

    Article  CAS  Google Scholar 

  • Vandevoorde L., Christiaens H., Verstraete W. (1992). Prevalence of coaggregation reactions among chicken lactobacilli. J. Appl. Bacteriol., 72: 214–219.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Belma Aslim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ekmekçi, H., Aslim, B. & Önal Darilmaz, D. Factors affecting the coaggregation ability of vaginal Lactobacilli withCandida spp.. Ann. Microbiol. 59, 163–167 (2009). https://doi.org/10.1007/BF03175615

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03175615

Key words

Navigation