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Identification ofGluconobacter strains isolated in Thailand based on 16S–23S rRNA gene ITS restriction and 16S rRNA gene sequence analyses

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Abstract

Forty-five acetic acid bacteria, which were isolated from fruits, flowers and other materials collected in Thailand by an enrichment culture approach, were assigned to the genusGluconobacter by phenotypic and chemotaxonomic characterisations. On the basis of 16S–23S rRNA gene ITS restriction and 16S rRNA gene sequence analyses, the forty-five isolates were grouped into five groups and identified at the specific level as follows: 1) seventeen isolates were grouped into Group A and identified asG. oxydans; 2) twelve isolates were grouped into Group B and identified asG. cerinus; 3) nine isolates were grouped into Group C and identified asG. frateurii; 4) six isolates were included into Group D and identified asG. thailandicus; 5) one isolate was grouped into Group E, characterised by a restriction pattern comprised of 667 and 48-bp fragments inAvaII digestion, differing from those of strains of Group C orG. frateurii and Group D orG. thailandicus, and unidentified.

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  1. Yuzo Yamada (JICA Senior Overseas Volunteer, Professor Emeritus)

    Present address: Shizuoka University, Shizuoka, Japan

Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, Chulalongkorn University, 10330, Bangkok, Thailand

    Jintana Kommanee & Ancharida Akaracharanya

  2. Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 10330, Bangkok, Thailand

    Somboon Tanasupawat

  3. BIOTEC Culture Collection (BCC), BIOTEC Central Research Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 12120, Pathumthani, Thailand

    Taweesak Malimas, Pattaraporn Yukphan & Yuzo Yamada (JICA Senior Overseas Volunteer, Professor Emeritus)

  4. NITE Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE), 292-0818, Kisarazu, Japan

    Yasuyoshi Nakagawa

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  1. Jintana Kommanee
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  2. Ancharida Akaracharanya
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Correspondence to Somboon Tanasupawat.

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Kommanee, J., Akaracharanya, A., Tanasupawat, S. et al. Identification ofGluconobacter strains isolated in Thailand based on 16S–23S rRNA gene ITS restriction and 16S rRNA gene sequence analyses. Ann. Microbiol. 58, 741–747 (2008). https://doi.org/10.1007/BF03175584

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  • DOI: https://doi.org/10.1007/BF03175584

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