Antimicrobial activity of seaweeds extracts against multiresistant pathogens
Fourteen seaweeds collected from the intertidal zone of Southwest coast of India were tested against ten human pathogen bacteria and one human pathogen fungus using the well diffusion test in the casitone agar medium. The species used in the present study include five Chlorophyta (Bryopsis plumosa, Ulva fasciata, Acrosiphonia orientalis, Chaetomorpha antennina, Grateloupia filicina), five Rhodophyta (Hypnea pannosa, Gracilaria corticata, Centroceras clavulatum, Portieria hornemannii, Cheilosporum spectabile) and four Phaeophyta (Padina tetrastromatica, Sargassum wightii, Stocheospermum marginatum, Chnoospora bicanaliculata). Of these, seven species were determined to be highly bioactive and screened on the multiresistant pathogens. We found that drying process has eliminated the active principles in the seaweeds. In the present study, methanol:toluene (3∶1) was found to be the best solvent for extracting the antimicrobial principles from fresh algae. However, the ethanolic extract showed no antibacterial activity.Acrosiphonia orientalis showed activity against 70% of the tested organisms.Stocheospermum marginatum was the only seaweed that showed activity againstKlebsiella pneumoniae. The extract fromGracilaria corticata was highly active againstProteus mirabilis, a Gram negative pathogenic bacterium. The present findings revealed that the tested seaweeds were highly active against Gram negative bacteria than Gram positive bacteria. The antimicrobial principle from seaweed was found to be a lipophilic compound. The compound was stable over a wide range of temperature (30–60 °C). The active principles of highly active seaweedsAcrosiphonia orientalis andStocheospermum marginatum were bactericidal.
Key wordsgreen algae brown algae red algae multiresistant pathogens antimicrobial activity
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- Egorov N.S. (1957). Determination of antibiotic activity of micro-organisms by the agar block method. Antibioki, 2: 50–53.Google Scholar
- Fables C.I., Arias A., Gill-Rodriguez M.C. (1995).In vitro study of antimicrobial activity in algae (Chlorophyta, Phaeophyta, Rhodophyta) collected from the coast of Tenerife (in Spanish). Anuario del Estudios Canneries, 34: 181–192.Google Scholar
- Fenical W., Paul V.S. (1984). Antimicrobial and cytotoxic terpenoids from tropical green algae of the family Udoteaceae. In: Bird C.J., Ragan M.A., Eds, 11th International Seaweed Symposium, Dr. W. Junk Publishers, Dordrccht, Boston, Lancaster, pp. 135–140.Google Scholar
- Forbes B.A., Sahm D.F., Weissfeld A.S., Trevino E.A. (1990). Methods for testing antimicrobial effectiveness. In: Baron E.J.et al., Eds, Bailey and Scott’ Diagnostic Microbiology, Mosby Co, St Louis, Missouri, pp. 171–194.Google Scholar
- Glombitza K.W. (1979). Antibiotics from algae. In: Hoppe I.L.A., Ed., Marine Algae in Pharmaceutical Science, Waiter de Gruyter, Berlin, New York, 303f.Google Scholar
- Harder R. (1917). Ernahrungphysiologische untersuchunghen an Cyanophyceen, Hauptsachlich am endophytischenNostoc punctiformie. Z. Bot., 9: 145.Google Scholar
- Lima-Filho J.V.M., Carvalho A.F.F.U., Freitas S.M. (2002). Antibacterial activity of extracts of six macroalgae from the Northeastern Brazillian coast. Brazilian J. Microbiol., 33: 311–333.Google Scholar
- Michanek G. (1979). Seaweed resources for pharmaceutical uses. In: Hoppe I.L.A., Ed., Marine Algae in Pharmaceutical Science, Waiter de Gruyter, Berlin, New York, pp. 203–235.Google Scholar
- Olessen P.E., Maretzki A., Almodovar L.A. (1963). An investigation of antimicrobial substances from marine algae. Bot. Mar., 6: 226–232.Google Scholar
- Selvin J. (2002) Shrimp disease management using secondary metabolites isolated from marine organisms. Ph.D dissertation, Manonmaniam Sundaranar University, Tirunelveli, India.Google Scholar
- Selvin J., Lipton A.P. (2004). Biopotentials ofUlva fasciata andHypnea musciformis collected from the peninsular coast of India. J. Mar. Sci. Technol., 12: 1–6.Google Scholar
- Tuney I, Cadirci BH, Unal D, Sukatar A. (2006). Antimicrobial activities of the extracts of marine algae from the coast of Urla (Imzir, Turkey). Turk. J. Biol., 30: 171–175.Google Scholar