Annals of Microbiology

, 59:465 | Cite as

First report ofAlternaria species associated with black point of wheat in Tunisia

  • Fatma Bensassi
  • Mouldi Zid
  • Ali Rhouma
  • Hassen Bacha
  • Mohamed Rabeh Hajlaoui
Ecological and Environmental Microbiology Short Communication


Durum wheat (Triticum turgidum) is one of the most important cereal crops in the world and the staple food for the Tunisian population. The main growing areas of durum wheat are located in the Northern part of the country, which is characterised by a sub humid climate favourable for fungal spoilage of grain such asAlternaria species. These species are able to produce plant pathogenic as well as toxic metabolites. For mycological analysis, the agar test was used. After mycelial growth, theAlternaria species were inoculated on potato carrot agar media. A PCR-based assay followed by a sequencing step allowed the identification ofAlternaria alternata; Alternaria tenuissima and Alternaria japonica from grains.

Key words

durum wheat Tunisia contamination Alternaria PCR ITS 


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Copyright information

© University of Milan and Springer 2009

Authors and Affiliations

  • Fatma Bensassi
    • 1
    • 2
  • Mouldi Zid
    • 1
  • Ali Rhouma
    • 3
  • Hassen Bacha
    • 2
  • Mohamed Rabeh Hajlaoui
    • 1
  1. 1.Laboratory of Plant Protection, the National Institute for Agricultural ResearchINRA TunisiaAriana
  2. 2.Laboratory for Research on Biologically Compatible CompoundsFaculty of DentistryMonastir
  3. 3.Research Unit of Plant Protection and EnvironmentOlive Tree Institute, Mahrajene CityTunisTunisia

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