Bacteriocins with anti-MRSA activity produced by water and soil isolated bacteria
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Among a thousand of bacteria isolated from forty-three samples, ten isolated bacteria strain WARY1-6, WARY9-1, WARY9-2, WARY6-6, SOPB1, WARY9-10, WARY7-4, WASM9-25, SOPB8-91 and WAS14 with antimicrobial activity against methicillin resistantStaphylococcus aureus (MRSA) were selected for further study. The activity of crude active supernatant (CAS) from these isolated bacteria was completely lost after treated with pronase E, chymotrypsin and trypsin demonstrating its proteinaceous nature. These isolated bacteria could be regarded as bacteriocin producing bacteria (BAC). It was also found that CAS from five Gram-positive isolated bacteria strain WARY1-6, WARY9-1, WARY9-2, WARY6-6 and WASM9-25 showed a broad range of inhibition as they can inhibit at least five Gram-positive and two Gram-negative test microorganisms. Two Gram-negative bacteria can be regarded as BAC with a broad range against both Gram-positive and Gram-negative test bacteria. These seven isolated bacteria can be regarded as BAC with a broad range of antagonistic activity. One isolated bacteria strain SOPB1 harboured a single large plasmid name pSOPB1-19. Its bacteriocin production was associated with plasmid as analysed by plasmid extraction and curing experiment. The strain SOPB1 was identified asBacillus sphaericus according to its 16s rRNA gene sequence. Its bacteriocin was heat stable up to 121 °C, 15 min and active within the pH range of 6–9.
Key wordswater and soil isolated bacteria anti-MRSA activity bacteriocin Bacillus sphaericus
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