Annals of Microbiology

, Volume 56, Issue 4, pp 393–398 | Cite as

Immunocytochemical characterisation of endophytic bacteriaAzospirillum brasilense, Herbaspirillum seropedicae, Burkholderia ambifaria andGluconacetobacter diazotrophicus

  • Antonella Canini
  • Lorena Canuti
  • Maria Grilli Caiola
  • Maddalena Del Gallo
Methods Original Articles


In the present work an immunocytochemical characterisation of four endophytic bacterial species has been made by using polyclonal antiserum produced against each of the four bacterial strains previously heated at 60 °C. The aim of this researchsito identify common elements among bacteria associated with their endophytic behaviour. Analysis of extracts of each strain by immunoblotting and ELISA confirmed the presence of proteins from different bacterial strains made up of common epitopes. However, antisaproduced againstHerbaspirillum seropedicae andBurkholderia ambifaria show a high number of bands recognised on each extracts, while antisera againstAzospirillum brasilense andGluconacetobacter diazotrophicus show a low number of bands recognised on each extract. Immunogold labelling showed that epitopes are located both on the cell wall and in the cytoplasm; most likely they could be preursor cell wall proteins synthesized inside the cytoplasm and subsequently transported onto cell wall. Finally, the common bands amog bacterial strains revealed by immunoblotting could play a role as active hydrolases involved in host tissue penetration.

Key words

endophytic bacteria immunogold labelling Western Blotting ELISA 


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Copyright information

© University of Milan and Springer 2006

Authors and Affiliations

  • Antonella Canini
    • 1
  • Lorena Canuti
    • 1
  • Maria Grilli Caiola
    • 1
  • Maddalena Del Gallo
    • 2
  1. 1.Dipartimento di BiologiaUniversità di Roma Tor VergataRomaItaly
  2. 2.Dipartimento di Biologia di Base e ApplicataUniversità dell’AquilaL’AquilaItaly

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