Annals of Microbiology

, 56:349 | Cite as

Detection of the intercellular adhesion loci (ica) in clinicalStaphylococcus aureus strains responsible for hospital acquired auricular infection

  • Tarek Zmantar
  • Kamel Chaieb
  • Hanene Miladi
  • Kacem Mahdouani
  • Amina Bakhrouf
Physiology and Metabolism Original Articles


In clinicalStaphylococcus aureus strains, the presence of theica genes, biofilm formation and susceptibility to antibiotics are considered important factors of virulence. In this study, 35 strains ofS. aureus, isolated from auricular infection, were investigated for slime production using Congo red agar (CRA) method, antibiotic susceptibility, presence ofmecA gene, and presence oficaA andicaD gene. The results show that 60% of strains weremecA positive when tested by PCR although 25.7% of strains were oxacillin resistant when tested with ATB STAPH. Qualitative slime production ofS. aureus using CRA revealed that 74.3% ofS. aureus were slime producers. All the strains carried theica gene.

Key words

Staphylococcus aureus slime production antibiotic susceptibility mecA gene ica loci 


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Copyright information

© University of Milan and Springer 2006

Authors and Affiliations

  • Tarek Zmantar
    • 1
  • Kamel Chaieb
    • 1
    • 2
  • Hanene Miladi
    • 1
  • Kacem Mahdouani
    • 2
  • Amina Bakhrouf
    • 1
  1. 1.Laboratoire d’Analyse et de Contrôle des Polluants Chimiques et Microbiologiques de l’Environnement à la Faculté de PharmacieMonastirTunisie
  2. 2.Laboratoire de Bactériologie de l’Hôpital Ibn El JazzarKairouanTunisie

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