Advertisement

Journal of Physiology and Biochemistry

, Volume 60, Issue 1, pp 31–37 | Cite as

Effects of dietary L-arginine supplementation on serum lipids and intestinal enzyme activities in diabetic rats

  • L. Míguez
  • G. Mariño
  • B. Rodríguez
  • C. Taboada
Article

Abstract

We investigated whether dietary supplementation with L-arginine, the endogenous precursor of nitric oxide, might affect serum lipid levels and activities of intestinal mucosa enzymes in animals, in which diabetes was induced by administration of streptozotocin. Control and diabetic rats were fed diets with or without 2% L-arginine supplementation for 4 weeks. Diabetic rats had significantly higher concentrations of serum triglycerides and LDL-cholesterol than control rats. These alterations were partially reduced by L-arginine supplementation. Experimental diabetes did not influence the lactase and leucine aminopeptidase activity in the intestine, but the activity of alkaline phosphatase was increased. Furthermore, activities of maltase and sucrase in the intestinal mucosa were elevated in streptozotocin-induced diabetic rats and were restored to control levels after dietary L-arginine supplementation. On the basis of the present experimental evidence, dietary L-arginine supplementation appears to affect the metabolism of lipoproteins and might alleviate some gastrointestinal dysfunctions, commonly seen in diabetes mellitus.

Key Words

Diabetes mellitus L-arginine Hyperlipemia Disaccharidases Alkaline phosphatase Leucine aminopeptidase 

Efecto de la suplementación dietética de Arg sobre los lípidos séricos y la actividad enzimática intestinal en ratas diabéticas

Resumen

En este trabajo se estudia si la suplementación de la dieta durante 4 semanas con un 2% de L-arginina, precursor endógeno del óxido nítrico, puede afectar al nivel de lípidos en sangre y a la actividad de algunas enzimas de la mucosa intestinal de animales diabéticos cuya diabetes fue inducida mediante la administración de estreptozotozina. Estos animales presentaron concentraciones significativamente más elevadas de triglicéridos y LDL-colesterol en suero que los controles, alteraciones que se redujeron ligeramente tras la administración de arginina. La diabetes experimental no afectó a la actividad de la lactasa ni de la leucino aminopeptidasa intestinales, pero aumentó el valor de la fosfatasa alcalina. Además, las actividades de la maltasa y sacarasa se vieron incrementadas en las ratas diabéticas, recuperándose los valores normales tras la administración de L-arginina. Parece, por tanto, que la administración de arginina como suplemento en la dieta influye en el metabolismo de las lipoproteínas y podría mejorar algunas disfunciones gastrointestinales, comunes en la diabetes mellitus.

Palabras clave

Diabetes mellitus L-arginina Hiperlipidemia Disacaridasas Fosfatasa alcalina Leucino aminopeptidasa 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Adawi, D., Molin, G. and Jeppsson, B. (1997):Scand. J. Gastroenterol.,3, 835–840.CrossRefGoogle Scholar
  2. 2.
    Al-Sawan, S. and Skillen, A. W. (1992):Cell. Biochem. Funct.,10, 261–266.CrossRefPubMedGoogle Scholar
  3. 3.
    Anjaneyulu, M. and Ramarao, P. (2002):Methods Find. Exp. Clin. Pharmacol.,24, 71–75.CrossRefPubMedGoogle Scholar
  4. 4.
    Appleton, J. (2002):Altern. Med. Rev.,7, 512–522.PubMedGoogle Scholar
  5. 5.
    Arvanitakis, C., Fanning, J. L. and Folscroft, J. (1980):Acta Diabetol. Lat.,17, 61–64.CrossRefPubMedGoogle Scholar
  6. 6.
    Avogaro, A., Toffolo, G., Kiwanuka, E., de Kreutzenberg, S. V., Tessari, P. and Cobelli, C. (2003):Diabetes,52, 795–802.CrossRefPubMedGoogle Scholar
  7. 7.
    Cassone, F. M., Laurenti, O., Desideri, G., Bravi, M. C., De Luca, O., Marinucci, M. C., De Mattia, G. and Ferri, C. (2002):Diabetologia,45, 1120–1127.CrossRefGoogle Scholar
  8. 8.
    Catanzaro, O. L., Marina-Prendes, M. G., S Hope, S. I., Zuccollo, A. and Dominguez, A. (1994):Braz. J. Med. Biol. Res.,27, 2043–2047.PubMedGoogle Scholar
  9. 9.
    Chandran, M., Chu, N. V. and Edelman, S. V. (2003):Curr. Diab. Rep.,3, 43–48.CrossRefPubMedGoogle Scholar
  10. 10.
    Dahlqvist, A. (1968):Anal. Biochem.,22, 99–107.CrossRefPubMedGoogle Scholar
  11. 11.
    Ersin, S., Tuncyurek, P., Esassolak, M., Alkanat, M., Buke, C., Yilmaz, M., Telefoncu, A. and Kose, T. (2000):J. Surg. Res.,89, 121–125.CrossRefPubMedGoogle Scholar
  12. 12.
    Gewalting, M. T. and Kodja, G. (2002):Cardiovasc. Res.,55, 250–260.CrossRefGoogle Scholar
  13. 13.
    Goldberg, J. A. and Ruttenberg, A. M. (1958):Cancer,2, 283–291. Marletta, M. A. (1993):J. Biol. Chem.,268, 12231–12234.CrossRefGoogle Scholar
  14. 14.
    Granstam, E. and Granstam, S. O. (2003):Physiol. Res.,52, 159–169.PubMedGoogle Scholar
  15. 15.
    Kessler, R. M., Acuto, O., Storelli, C., Murer, H., Muller, M. and Semenza, G. A. (1978):Biochim. Biophys. Acta,506, 136–154.CrossRefPubMedGoogle Scholar
  16. 16.
    Kim, H. and Hwan Kim, K. (2001):Pharmacology,62, 200–207.CrossRefPubMedGoogle Scholar
  17. 17.
    Kurowska, E. M. (2002):Curr. Pharm. Des.,8, 155–166.CrossRefPubMedGoogle Scholar
  18. 18.
    Kurowska, E. M. and Carrol, K. K. (1998):Biochim. Biophys. Acta,1392, 41–50.PubMedGoogle Scholar
  19. 19.
    Lekakis, J. P., Papathanassiou, S., Papaioannou, T. G., Papamichael, C. M., Zakapoulos, N., Dagre, A. G., Stamatelopoulos, K., Protogerou, A. and Stamatelopoulos, S. F. (2002):Int. J. Cardiol. 86, 317–323.CrossRefPubMedGoogle Scholar
  20. 20.
    Llorens, S., Jordán, J. and Nava, E. (2002):J. Physiol. Biochem.,58, 179–188.PubMedGoogle Scholar
  21. 21.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randam, R. J. (1951):J. Biol. Chem. 193, 267–275.Google Scholar
  22. 22.
    Miller, D. L. (1981):Am. J. Clin. Nutr.,34, 1153–1170.PubMedGoogle Scholar
  23. 23.
    Mendez, J. D. and Balderas, F. (2001):Biochimie,83, 453–458.CrossRefPubMedGoogle Scholar
  24. 24.
    Murakami, I. and Ikeda, T. (1998):Scand. J. Gastroenterol.,33, 1069–1073.CrossRefPubMedGoogle Scholar
  25. 25.
    Myers, P. R., Wright, T. F., Tanner, M. A. and Ostlund, R. E. (1994):J. Lab. Clin. Med.,12 672–683.Google Scholar
  26. 26.
    Nakabou, Y., Ikeuchi, K., Minami, H. and Hagihira, H. (1985):Experientia,15, 482–484.CrossRefGoogle Scholar
  27. 27.
    Ohta, Y. and Nishida, K. (2002):Clin. Exp. Pharmacol. Physiol.,29, 32–38.CrossRefPubMedGoogle Scholar
  28. 28.
    Popov, D., Costache, G., Georgescu, A. and Enache, M. (2002),Cell Tissue Res.,308, 109–1120.CrossRefPubMedGoogle Scholar
  29. 29.
    Ramaswamy, K. and Flint, P. W. (1980):Am. J. Physiol. 238, G114-G118.PubMedGoogle Scholar
  30. 30.
    Schedl, H. P., Al-Jurf, A. S. and Wilson, H. D. (1983):Diabetes,32, 265–270CrossRefPubMedGoogle Scholar
  31. 31.
    Takenoshita, M., Yamaji, R., Inui, H., Miyatake, K. and Nakano, Y. (1998):Biochem. J.,329, 597–600.PubMedGoogle Scholar
  32. 32.
    Tormo, M. A., Martínez, I. M., Romero de Tejada, A., Gil-Exojo, I. and Campillo, J. E. (2002):Exp. Clin. Endocrinol. Diabetes,110, 119–123.CrossRefPubMedGoogle Scholar
  33. 33.
    Tousoulis, D., Antoniades, C., Tentolouris, C. Goumas, G., Stefanadiis, C. And Toutouzas, P. (2002):Vasc. Med.,7, 203–211.CrossRefPubMedGoogle Scholar
  34. 34.
    Unakami, S., Komoda, T. and Sakagishi, Y. (1990):Int. J. Biochem.,22, 1325–1331.CrossRefPubMedGoogle Scholar
  35. 35.
    Wild, G. E., Thompson, J. A., Searles, L., Turner, R., Hasan, J. and Thomson, A. B. (1999):Dig. Dis. Sci.,44, 407–414.CrossRefPubMedGoogle Scholar
  36. 36.
    Wink, D. A. and Mitchell, J. B. (1998):Free. Rad. Biol. Med.,25, 434–456.CrossRefPubMedGoogle Scholar
  37. 37.
    Wu, G. and Meininger, C. J. (2000):J. Nutr.,130, 2626–2629.PubMedGoogle Scholar
  38. 38.
    Zalba, G., Beaumont, J., San Jose, G., Fortuño, A., Fortuño, M. A. and Díez, J. (2000):J. Physiol. Biochem. 56, 57–64.PubMedCrossRefGoogle Scholar

Copyright information

© Universidad de Navarra 2004

Authors and Affiliations

  • L. Míguez
    • 1
  • G. Mariño
    • 1
  • B. Rodríguez
    • 1
  • C. Taboada
    • 1
  1. 1.Department of Physiology, Faculty of PharmacyUniversity of Santiago de CompostelaSantiago de CompostelaSpain

Personalised recommendations