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Applied Magnetic Resonance

, 28:411 | Cite as

Detection of free radicals formation in haemolymph of insects by EPR spectroscopy

  • D. A. Komarov
  • I. A. Slepneva
  • V. V. Glupov
  • V. V. Khramtsov
Article

Abstract

The generation of reactive oxygen species during oxidation of DOPA (3-(3,4-dihydroxyphenyl-DL-alanine) in haemolymph of insects has been studied by electron paramagnetic resonance with spin traps 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide and 1-hydroxy-3-carboxy-pyrrolidine. The data support the formation of DOPA-derived highly reactive intermediates during melanization in haemolymph of insects. The formation of superoxide has not been detected apparently due to reaction of DOPA with superoxide. The rate constant of the latter reaction was estimated ask≈5·105 M−1.c−1. The formation of DOPA-semiquinone in haemolymph has been measured using spin stabilization ofo-semiquinones by Mg2+. The comparative studies in haemolymph of intact insects and insects infected by fungal infection suggest an important role of DOPA-semiquinone production in the immune status of insects.

Keywords

Electron Paramagnetic Resonance Electron Paramagnetic Resonance Spectrum Dopa Electron Paramagnetic Resonance Signal Electron Paramagnetic Resonance Spectroscopy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer 2005

Authors and Affiliations

  • D. A. Komarov
    • 1
  • I. A. Slepneva
    • 1
  • V. V. Glupov
    • 2
  • V. V. Khramtsov
    • 1
    • 3
  1. 1.Institute of Chemical Kinetics and CombustionRussian Academy of SciencesNovosibirskRussian Federation
  2. 2.Institute of Animal Systematics and EcologyRussian Academy of SciencesNovosibirskRussian Federation
  3. 3.Dorothy M. Davis Heart and Lung Research InstituteThe Ohio State UniversityColumbusUSA

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