Applied Magnetic Resonance

, Volume 31, Issue 3–4, pp 387–410 | Cite as

Saturation transfer EPR studies of slow rotational motion in membranes



Saturation transfer spectroscopy is a nonlinear electron paramagnetic resonance method for detecting rotational diffusion of nitroxide spin labels on the microsecond timescale or slower. Various results on spin-labeled biological membranes are reviewed here. These studies include those on lipid motions in gel-phase phospholipid bilayers, on the dynamics of lipid-protein interactions, and on the rotational diffusion of spin-labeled transmembrane proteins. Certain methodological aspects are also discussed.


Electron Paramagnetic Resonance Nitroxide Rotational Diffusion Rotational Correlation Time Rotational Mobility 


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Copyright information

© Springer 2007

Authors and Affiliations

  1. 1.Abteilung SpektroskopieMax-Planck-Institut für Biophysikalische ChemieGöttingenGermany

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