Applied Magnetic Resonance

, Volume 31, Issue 3–4, pp 387–410 | Cite as

Saturation transfer EPR studies of slow rotational motion in membranes

  • D. Marsh


Saturation transfer spectroscopy is a nonlinear electron paramagnetic resonance method for detecting rotational diffusion of nitroxide spin labels on the microsecond timescale or slower. Various results on spin-labeled biological membranes are reviewed here. These studies include those on lipid motions in gel-phase phospholipid bilayers, on the dynamics of lipid-protein interactions, and on the rotational diffusion of spin-labeled transmembrane proteins. Certain methodological aspects are also discussed.


Electron Paramagnetic Resonance Nitroxide Rotational Diffusion Rotational Correlation Time Rotational Mobility 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Marsh D., Watts A. in: Lipid-Protein Interactions (Jost P.C., Griffith O.H., eds.), vol. 2 pp. 53–126. New York: Wiley-Interscience 1982.Google Scholar
  2. 2.
    Esmann M., Sar P.C., Hideg K., Marsh D.: Anal. Biochem.213, 336–348 (1993)CrossRefGoogle Scholar
  3. 3.
    Esmann M., Hankovszky H.O., Hideg K., Pedersen J.A., Marsh D.: Anal. Biochem.189, 274–282 (1990)CrossRefGoogle Scholar
  4. 4.
    Marchetto R., Schreier S., Nakaie C.R.: J. Am. Chem. Soc.115, 11042–11043 (1993)CrossRefGoogle Scholar
  5. 5.
    Esmann M., Hankovszky H.O., Hideg K., Marsh D.: Biochim. Biophys. Acta978, 209–215 (1989)CrossRefGoogle Scholar
  6. 6.
    Horváth L.I., Dux L., Hankovszky H.O., Hideg K., Marsh D.: Biophys. J.58, 231–241 (1990)CrossRefGoogle Scholar
  7. 7.
    Esmann M., Hideg K., Marsh D.: Biochemistry33, 3693–3697 (1994)CrossRefGoogle Scholar
  8. 8.
    Monaco V., Formaggio F., Crisma M., Toniolo C., Hanson P., Millhauser G.L.: Biopolymers50, 239–253 (1999)CrossRefGoogle Scholar
  9. 9.
    Marsh D.: J. Magn. Reson.180, 305–310 (2006)CrossRefADSGoogle Scholar
  10. 10.
    Cherry R.J., Godfrey R.E.: Biophys. J.36, 257–276 (1981)CrossRefADSGoogle Scholar
  11. 11.
    Jähnig F.: Eur. Biophys. J.14, 63–64 (1986)CrossRefGoogle Scholar
  12. 12.
    Thomas D.D., Dalton L.R., Hyde J.S.: J. Chem. Phys.65, 3006–3024 (1976)CrossRefADSGoogle Scholar
  13. 13.
    Marsh D., Horváth L.I.: J. Magn. Reson.99, 323–331 (1992)Google Scholar
  14. 14.
    Marsh D.: Appl. Magn. Reson.3, 53–65 (1992)ADSCrossRefGoogle Scholar
  15. 15.
    Horváth L.I., Marsh D.: J. Magn. Reson.80, 314–317 (1988)Google Scholar
  16. 16.
    Robinson B.H., Dalton L.R.: J. Chem. Phys.72, 1312–1324 (1980)CrossRefADSGoogle Scholar
  17. 17.
    Beth A.H., Hustedt E.J. in: Biomedical EPR, part B (Eaton G.R., Eaton S.S., Berliner L.J., eds.). Biological Magnetic Resonance, vol. 24, pp. 369–407. New York: Kluwer 2005.Google Scholar
  18. 18.
    Beth A.H., Robinson B.H. in: Spin Labeling: Theory and Applications (Berliner L.J., Reuben J., eds.). Biological Magnetic Resonance, vol. 8, pp. 179–253. New York: Plenum 1989.Google Scholar
  19. 19.
    Fajer P., Marsh D.: J. Magn. Reson.51, 446–459 (1983)Google Scholar
  20. 20.
    Fajer P., Watts A., Marsh D.: Biophys. J.61, 879–891 (1992)CrossRefADSGoogle Scholar
  21. 21.
    Watts A., Marsh D.: Biochim. Biophys. Acta642, 231–241 (1981)CrossRefGoogle Scholar
  22. 22.
    Marsh D.: Biochemistry19, 1632–1637 (1980)CrossRefGoogle Scholar
  23. 23.
    Marsh D., Watts A.: Biochem. Biophys. Res. Commun.94, 130–137 (1980)CrossRefGoogle Scholar
  24. 24.
    Horváth L.I., Marsh D.: J. Magn. Reson.54, 363–373 (1983)Google Scholar
  25. 25.
    Marsh D., Horváth L.I.: J. Magn. Reson.97, 13–26 (1992)Google Scholar
  26. 26.
    Páli T., Bartucci R., Horváth L.I., Marsh D.: Biophys. J.64, 1781–1788 (1993)CrossRefGoogle Scholar
  27. 27.
    Páli T., Bartucci R., Horváth L.I., Marsh D.: Biophys. J.61, 1595–1602 (1992)CrossRefGoogle Scholar
  28. 28.
    Bartucci R., Páli T., Marsh D.: Biochemistry32, 274–281 (1993)CrossRefGoogle Scholar
  29. 29.
    Marsh D., Horváth L.I.: Biochim. Biophys. Acta1376, 267–296 (1998)Google Scholar
  30. 30.
    Horváth L.I., Brophy P.J., Marsh D.: Biophys. J.64, 622–631 (1993)CrossRefGoogle Scholar
  31. 31.
    Li G., Horváth L.I., Knowles P.F., Murphy D.J., Marsh D.: Biochim. Biophys. Acta987, 187–192 (1989)CrossRefGoogle Scholar
  32. 32.
    Kleinschmidt J.H., Powell G.L., Marsh D.: Biochemistry37, 11579–11585 (1998)CrossRefGoogle Scholar
  33. 33.
    Páli T., Kleinschmidt J.H., Powell G.J., Marsh D.: Biochemistry39, 2355–2361 (2000)CrossRefGoogle Scholar
  34. 34.
    Marsh D. in: Spin Labeling: Theory and Applications (Berliner, L.J., Reuben J., eds.). Biological Magnetic Resonance vol. 8, pp., 255–303. New York: Plenum 1989.Google Scholar
  35. 35.
    Horváth L.I., Brophy P.J., Marsh D.: Biochim. Biophys. Acta1147, 277–280 (1993)CrossRefGoogle Scholar
  36. 36.
    Brophy P.J., Horváth L.I., Marsh D.: Biochemistry23, 860–865 (1984)CrossRefGoogle Scholar
  37. 37.
    Arora A., Esmann M., Marsh D.: Biochemistry38, 10084–10091 (1999)CrossRefGoogle Scholar
  38. 38.
    Livshits V.A., Páli T., Marsh D.: J. Magn. Reson.133, 79–91 (1998)CrossRefADSGoogle Scholar
  39. 39.
    Livshits V.A., Dzikovski B.G., Marsh D.: J. Magn. Reson.162, 429–442 (2003)CrossRefADSGoogle Scholar
  40. 40.
    Hustedt E.J., Beth A.H.: Biophys. J.69, 1409–1423 (1995)CrossRefADSGoogle Scholar
  41. 41.
    Hebert H., Purhonen P., Vorum H., Thomsen K., Maunsbach A.B.: J. Mol. Biol.314, 479–494 (2001)CrossRefGoogle Scholar
  42. 42.
    Esmann M., Horváth L.I., Marsh D.: Biochemistry26, 8675–8683 (1987)CrossRefGoogle Scholar
  43. 43.
    Nury H., Dahout-Gonzalez C., Trézéguet V., Lauquin G., Brandolin G., Pebay-Peyroula E.: FEBS Lett.579, 6031–6036 (2005)CrossRefGoogle Scholar
  44. 44.
    Horváth L.I., Munding A., Beyer K., Klingenberg M., Marsh D.: Biochemistry28, 407–414 (1989)CrossRefGoogle Scholar
  45. 45.
    Fei M.J., Yamashita E., Inoue N., Yao M., Yamaguchi H., Tsukihara T., Shinzawa-Itoh K., Nakashima R., Yoshikawa S.: Acta Crystallogr. D56, 529–535 (2000)CrossRefGoogle Scholar
  46. 46.
    Fajer P., Knowles P.F., Marsh D.: Biochemistry28, 5634–5643 (1989)CrossRefGoogle Scholar
  47. 47.
    Toyoshima C., Nakasako M., Nomura N., Ogawa H.: Nature405, 647–655 (2000)CrossRefADSGoogle Scholar
  48. 48.
    de Jongh H.H.J., Hemminga M.A., Marsh D.: Biochim. Biophys. Acta1024, 82–88 (1990)CrossRefGoogle Scholar
  49. 49.
    Teller D.C., Okada T., Behnke C.A., Palczewski K., Stenkamp R.E.: Biochemistry40, 7761–7772 (2001)CrossRefGoogle Scholar
  50. 50.
    Ryba N.J.P., Marsh D.: Biochemistry31, 7511–7518 (1992)CrossRefGoogle Scholar
  51. 51.
    Abramson J., Smirnova I., Kasho V., Verner G., Kaback H.R., Iwata S.: Science,301, 610–615 (2003)CrossRefADSGoogle Scholar
  52. 52.
    Marsh D., Henderson P.J.F.: Biochim. Biophys. Acta,1510, 464–473 (2001)CrossRefGoogle Scholar
  53. 53.
    Páli T., Finbow M.E., Holzenburg A., Findlay J.B.C., Marsh D.: Biochemistry34, 9211–9218 (1995)CrossRefGoogle Scholar
  54. 54.
    Páli T., Whyteside G., Dixon N., Kee T.P., Ball S., Harrison M.A., Findlay J.B.C., Finbow M.E., Marsh D.: Biochemistry43, 12297–12305 (2004)CrossRefGoogle Scholar
  55. 55.
    Páli T., Finbow M.E., Marsh D.: Biochemistry38, 14311–14319 (1999)CrossRefGoogle Scholar
  56. 56.
    Fox Jr. R.O., Richards F.M.: Nature300, 325–330 (1982)CrossRefADSGoogle Scholar
  57. 57.
    Marsh D., Jost M., Peggion C., Toniolo C.: Biophys. J. (2007) in press.Google Scholar
  58. 58.
    Marsh D.: Eur. Biophys. J.26, 203–208 (1997)CrossRefGoogle Scholar
  59. 59.
    Knowles P.F., Watts A., Marsh D.: Biochemistry18, 4480–4487 (1979)CrossRefGoogle Scholar
  60. 60.
    Páli T., Bashtovyy D., Marsh D.: Protein Sci.15, 1153–1161 (2006)CrossRefGoogle Scholar
  61. 61.
    Powell G.L., Knowles P.F., Marsh D.: Biochemistry29, 5127–5132 (1990)CrossRefGoogle Scholar
  62. 62.
    Cone R.A.: Nature236, 39–43 (1972)Google Scholar
  63. 63.
    Downer N.W.: Biophys. J.47, 285–293 (1985)CrossRefADSGoogle Scholar
  64. 64.
    Fotiadis D., Liang Y., Filipek S., Saperstein D.A., Engel A., Palczewski K.: Nature421, 127–128 (2003)CrossRefADSGoogle Scholar
  65. 65.
    Nagle J.F., Tristram-Nagle S.: Biochim. Biophys. Acta1469, 159–195 (2000)Google Scholar
  66. 66.
    Lomize M.A., Lomize A.L., Pogozheva I., Mosberg H.I.: Bioinformatics22, 623–625 (2006)CrossRefGoogle Scholar
  67. 67.
    Ryba N.J.P., Hoon M.A., Findlay J.B.C., Saibil H.R., Wilkinson J.R., Heimburg T., Marsh D.: Biochemistry32, 3298–3305 (1993)CrossRefGoogle Scholar
  68. 68.
    Venien-Bryan C., Davies A., Langmack K., Baverstock J., Watts A., Marsh D., Saibil H.: FEBS Lett.359, 45–49 (1995)CrossRefGoogle Scholar
  69. 69.
    Marsh D.: Handbook of Lipid Bilayers. Boca Raton, Fla.: CRC Press 1990.Google Scholar
  70. 70.
    Esmann M., Karlish S.J.D., Sottrup-Jensen L., Marsh D.: Biochemistry33, 8044–8050 (1994)CrossRefGoogle Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  1. 1.Abteilung SpektroskopieMax-Planck-Institut für Biophysikalische ChemieGöttingenGermany

Personalised recommendations