Applied Magnetic Resonance

, 22:497 | Cite as

EPR study of the toxicological effects of gas-phase cigarette smoke and the protective effects of grape seed extract on the mitochondrial membrane

  • J. Gao
  • H. Tang
  • Y. Li
  • H. Liu
  • B. Zhao


Electron paramagnetic resonance spin trapping and spin labeling techniques were used to study the effects of grape seed extract on the biophysical propertiers of the mitochondrial membrane exposed to gas-phase cigarette smoke. The spin trapping results indicated that grape seed extract can effectively scavenge free radicals in cigarette smoke and the lipid free radicals generated from the lipid peroxidation of mitochondrial membrane caused by gas-phase cigarette smoke. The spin labeling results showed that grape seed extract can protect the mitochondrial membrane from the toxicological damage caused by gas-phase cigarette smoke.


Electron Paramagnetic Resonance Mitochondrial Membrane Catechin EGCG Electron Paramagnetic Resonance Spectrum 
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Copyright information

© Springer 2002

Authors and Affiliations

  • J. Gao
    • 1
  • H. Tang
    • 2
  • Y. Li
    • 3
  • H. Liu
    • 3
  • B. Zhao
    • 1
  1. 1.Laboratory of Visual Information Processing, Department of Molecular and Cell Biophysics, Institute of BiophysicsAcademia SinicaBeijingPeople’s Republic of China
  2. 2.Institute of Food ResearchNorwichUK
  3. 3.Department of ChemistryPeking UniversityBeijingChina

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