An experimental model of thyroid cancer was prepared for evaluating the accumulation of [14C]deoxy-D-glucose ([14C]DG) in thyroid cancer xenografts (AC2). A continuous cell line established from a biopsy specimen of a metastatic thyroid carcinoma possessed the ability to synthesize the cellular protein without increase in cell division after adding bovine TSHin vitro. The histological sections of the xenografts resected from the131I treated nude mice mainly consisted of structures showing follicular and trabecular growth. Immunohistochemically the cytoplasm of the tumor cells was positive for human thyroglobulin(hTg). These observations provide strong evidence that the AC2 cell originates in the thyroid follicular epithelium. By comparing autoradiographic accumulation patterns of [14C]DG and histopathological examinations, it was found that the uptake of [14C]DG was higher in the granulation tissues surrounding necrosis than in viable tumor cells of trabeculary growing and follicle forming tissues.
It is suggested that the degree of [14C]DG content reflects not only tumor cell viability and proliferation but also the inflammatory and degenerative reaction accompanying tumor cell growth.
Paul R. Comparison of fluorine-18-2-fiuorodeoxyglucose and gallium-67 citrate imaging for detection of lymphoma.J Nucl Med 28: 288–292, 1987.PubMedGoogle Scholar
Nolop KB, Rhodes CG, Brudin LH, et al. Glucose utilizationin vivo by human pulmonary neoplasms.Cancer 60: 2682–2689, 1987.PubMedCrossRefGoogle Scholar
Joensuu H, Ahonen A. Imaging of metastases of thyroid carcinoma with fluorine-18 fluorodeoxyglucose.J Nucl Med 28: 910–914, 1987.PubMedGoogle Scholar
Kopriwa BM, Leblond CP. Improvements in the coating technique of radioautography.J Histochem Cytochem 10: 269–284, 1962.Google Scholar
Carcangiu ML, Steeper T, Zampi G, et al. Anaplastic carcinoma—A study of 70 cases.Am J Clin Pathol 83: 135–158, 1985.PubMedGoogle Scholar
Reivich M, Kuhl D, Wolf A, et al. The18F-2-fluolo-2-deoxy-D-glucose method for the measurement of local cerebral glucose utilization in man.Circ Res 44: 127–137, 1979.PubMedGoogle Scholar
Tsuda T.In vitro andin vivo characterizations of established human follicular carcinoma cell line derived from thyroid cancer: A novel model for well-differentiated thyroid malignant tumor.Ann Nucl Med 6: 159–168, 1992.PubMedCrossRefGoogle Scholar
Valenta L, Kyncl F, Niederle B, Jirousek L. Soluble proteins in thyroid neoplasia.J Clin Endocrinol Metab 28: 442–450, 1968.PubMedCrossRefGoogle Scholar
Leche AR, Staub J, Kohler-Faden R, Muller-Brand J, et al. Thyroglobulin production by malignant thyroid tumors: An immunocytochemical and radioimmunoassay study.Cancer 57: 1145–1153, 1986.CrossRefGoogle Scholar
Minn H, Joensuu H, Ahonen A, Klemi P. Fluorodeoxyglucose imaging: a method to assess the proliferative activity of human cancerin vivo. Comparison with DNA flow cytometry in head and neck tumors.Cancer 61: 1776–1781, 1988.PubMedCrossRefGoogle Scholar
Kubota R, Yamada S, Kubota K, et al. Intratumoral distribution of fluorine-18-fluorodeoxyglucosein vivo: High accumulation in macrophages and granulation tissues studied by microautoradiography.J Nucl Med 33: 1972–1980, 1992.PubMedGoogle Scholar