Annals of Nuclear Medicine

, Volume 8, Issue 1, pp 75–83 | Cite as

Autoradiographic analysis of [14C]deoxy-D-glucose in thyroid cancer xenografts: A comparative study with pathologic correlation

  • Masahiro Kubota
  • Takatoshi Tsuda
  • Takashi Minase
  • Kunihiro Nakada
  • Masayori Furudate
Short Communication


An experimental model of thyroid cancer was prepared for evaluating the accumulation of [14C]deoxy-D-glucose ([14C]DG) in thyroid cancer xenografts (AC2). A continuous cell line established from a biopsy specimen of a metastatic thyroid carcinoma possessed the ability to synthesize the cellular protein without increase in cell division after adding bovine TSHin vitro. The histological sections of the xenografts resected from the131I treated nude mice mainly consisted of structures showing follicular and trabecular growth. Immunohistochemically the cytoplasm of the tumor cells was positive for human thyroglobulin(hTg). These observations provide strong evidence that the AC2 cell originates in the thyroid follicular epithelium. By comparing autoradiographic accumulation patterns of [14C]DG and histopathological examinations, it was found that the uptake of [14C]DG was higher in the granulation tissues surrounding necrosis than in viable tumor cells of trabeculary growing and follicle forming tissues.

It is suggested that the degree of [14C]DG content reflects not only tumor cell viability and proliferation but also the inflammatory and degenerative reaction accompanying tumor cell growth.

Key words

thyroid carcinoma xenograft [14C]deoxy-D-glucose ([14C]DG) autoradiography 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Weber G, Morris HP. Comparative biochemistry of hepatomas. III. Carbohydrate enzymes in liver tumors of different growth rate.Cancer Res 23: 987–993, 1963.PubMedGoogle Scholar
  2. 2.
    Burk D, Wood M, Hunter J. On the significance of glycolysis for cancer growth with special reference to Morris rat hepatomas.J Natl Cancer Inst 38: 839–863, 1967.PubMedGoogle Scholar
  3. 3.
    Watanabe A, Tanaka R, Takeda N, Washiyama K. DNA synthesis, blood flow, and glucose utilization in experimental rat brain tumors.J Neurosurg 70: 86–91, 1989.PubMedCrossRefGoogle Scholar
  4. 4.
    Strauss LG, Conti PS. The applications of PET in clinical oncology.J Nucl Med 32: 623–648, 1991.PubMedGoogle Scholar
  5. 5.
    Di Chiro G, DeLaPaz RL, Brooks RA, et al. Glucose utilization of cerebral gliomas measured by [18F]-fluorodeoxy glucose and positron emission tomograpy.Neurology 32: 1323–1329, 1982.PubMedGoogle Scholar
  6. 6.
    Paul R, Ahonen A, Nordman E. Imaging of hepatoma with [18F]fluorodeoxyglucose.Lancet 1: 50–51, 1985.PubMedCrossRefGoogle Scholar
  7. 7.
    Paul R. Comparison of fluorine-18-2-fiuorodeoxyglucose and gallium-67 citrate imaging for detection of lymphoma.J Nucl Med 28: 288–292, 1987.PubMedGoogle Scholar
  8. 8.
    Nolop KB, Rhodes CG, Brudin LH, et al. Glucose utilizationin vivo by human pulmonary neoplasms.Cancer 60: 2682–2689, 1987.PubMedCrossRefGoogle Scholar
  9. 9.
    Joensuu H, Ahonen A. Imaging of metastases of thyroid carcinoma with fluorine-18 fluorodeoxyglucose.J Nucl Med 28: 910–914, 1987.PubMedGoogle Scholar
  10. 10.
    Kopriwa BM, Leblond CP. Improvements in the coating technique of radioautography.J Histochem Cytochem 10: 269–284, 1962.Google Scholar
  11. 11.
    Carcangiu ML, Steeper T, Zampi G, et al. Anaplastic carcinoma—A study of 70 cases.Am J Clin Pathol 83: 135–158, 1985.PubMedGoogle Scholar
  12. 12.
    Reivich M, Kuhl D, Wolf A, et al. The18F-2-fluolo-2-deoxy-D-glucose method for the measurement of local cerebral glucose utilization in man.Circ Res 44: 127–137, 1979.PubMedGoogle Scholar
  13. 13.
    Tsuda T.In vitro andin vivo characterizations of established human follicular carcinoma cell line derived from thyroid cancer: A novel model for well-differentiated thyroid malignant tumor.Ann Nucl Med 6: 159–168, 1992.PubMedCrossRefGoogle Scholar
  14. 14.
    Valenta L, Kyncl F, Niederle B, Jirousek L. Soluble proteins in thyroid neoplasia.J Clin Endocrinol Metab 28: 442–450, 1968.PubMedCrossRefGoogle Scholar
  15. 15.
    Leche AR, Staub J, Kohler-Faden R, Muller-Brand J, et al. Thyroglobulin production by malignant thyroid tumors: An immunocytochemical and radioimmunoassay study.Cancer 57: 1145–1153, 1986.CrossRefGoogle Scholar
  16. 16.
    Minn H, Joensuu H, Ahonen A, Klemi P. Fluorodeoxyglucose imaging: a method to assess the proliferative activity of human cancerin vivo. Comparison with DNA flow cytometry in head and neck tumors.Cancer 61: 1776–1781, 1988.PubMedCrossRefGoogle Scholar
  17. 17.
    Kubota R, Yamada S, Kubota K, et al. Intratumoral distribution of fluorine-18-fluorodeoxyglucosein vivo: High accumulation in macrophages and granulation tissues studied by microautoradiography.J Nucl Med 33: 1972–1980, 1992.PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Masahiro Kubota
    • 1
    • 4
  • Takatoshi Tsuda
    • 1
    • 5
  • Takashi Minase
    • 2
  • Kunihiro Nakada
    • 3
  • Masayori Furudate
    • 3
  1. 1.Department of Radiology, School of MedicineSapporo Medical UniversityJapan
  2. 2.Clinical Laboratory of NTT Sapporo HospitalJapan
  3. 3.Department of Nuclear MedicineFaculty of Medicine, Hokkaido UniversityJapan
  4. 4.Department of RadiologyTeine Keijinkai HospitalSapporoJapan
  5. 5.Department of RadiologyKushiro Chuo HospitalJapan

Personalised recommendations