Abstract
The conformation in solution of new modified nucleosides bearing branched-chain sugars used as building blocks for antisense oligonucleotides is investigated by NMR spectroscopy. Within a classical approach to the conformational study of these molecules, a new methodology is adopted, which is capable of exploring all the possibilities, for a given conformational model, that are compatible with the experimental data. The relationship between conformation of the monomers and hybridization properties of the corresponding oligonucleotides is examined.
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Cicero, D.O., Iribarren, A.M. & Bazzo, R. Conformational analysis by NMR spectroscopy of 2′-deoxy-2′-C-alkylnucleosides: Building blocks of new antisense fragments. Appl. Magn. Reson. 7, 95–105 (1994). https://doi.org/10.1007/BF03162550
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DOI: https://doi.org/10.1007/BF03162550