Abstract
A new variant of lipoxygenases, one containing manganese instead of iron, is characterized by electron paramagnetic resonance (EPR) at two frequencies. In the manganous state (Se = 5/2), maganese lipoxygenase (MnLO) yields very broad X-band (9.2 GHz) EPR signals, extending over about 800 mT. In contrast, at W-band (94 GHz), the signal is much simplified, consisting of nested transitions centered near the free electrong-value. Computer simulation has been employed to derive estimates of the zero-field splittings for MnLO, with data from these two EPR frequencies. The general features of both X- and W-band spectra are fit, first, by simulations with Se = 5/2, but no nuclear hyperfine splitting. The simulations are then refined by inclusion of the hyperfine splitting. On the basis of the simulations, the ranges of zero-field splitting parameters areD = +0.07 to +0.10 cm−1, andE/D = 0.13 to 0.23. Comparison of the value ofD for MnLO with that of other manga-nese-containing proteins suggests that MnLO has three N-ligands to the metal center and O-ligands in the remainder of 6 coordination positions. The coordination environment of MnLO is similar to that in iron lipoxygenases.
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An erratum to this article is available at http://dx.doi.org/10.1007/BF03166817.
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Gaffney, B.J., Su, C. & Oliw, E.H. Assignment of EPR transitions in a manganese-containing lipoxygenase and prediction of local structure. Appl. Magn. Reson. 21, 411–422 (2001). https://doi.org/10.1007/BF03162417
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DOI: https://doi.org/10.1007/BF03162417