Area-specific modification of acetylcholinesterase activity following 3-mercaptopropionic acid-induced seizures
- 27 Downloads
Acetylcholinesterase activity (AChE) was assayed in rat CNS membrane fractions after administration of the convulsant 3-mercaptopropionic acid (150 mg/kg, ip). In comparison with saline-injected controls, total AChE activity decreased 12–20% in striatum and cerebellum during seizure and postseizure but failed, to change in cerebral cortex. Specific AChE activity, assayed in the presence of 10−4 M ethopropazine (a butyrylcholinesterase inhibitor) decreased 15–25% in striatum and cerebellum, increased 20–45% in hippocampus, but remained unchanged in cerebral cortex. Saline injection alone increased AChE activity in striatum (68%) and cerebellum (36%) but failed to modify enzyme activity in hippocampus and cerebral cortex. To conclude, AChE sensitivity to convulsant, and saline administration is tissue-specific and not restricted to cholinergic areas.
Index EntriesAcetylcholinesterase seizures 3-mercaptopropionic acid striatum cerebellum hippocampus saline injection convulsant
Unable to display preview. Download preview PDF.
- Emson P. C. (1978) Biochemical and metabolical changes in epilepsy, in:Taurine and Neurological Disorders (Barbeau A. and Huxtable R. J., eds.), pp. 319–338, Raven, New York.Google Scholar
- Wasterlain C. G., Farber D. B., and Fairchild M. D. (1986) Synaptic mechanism in the kindled epileptic focus; a speculative synthesis. inAdvances in Neurology (Delgado-Escueta A. V., Ward A. A. Jr., Woodbury D. M., and Porter R. J., eds.), pp. 411–433, Raven, New York.Google Scholar