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Detergent effects on an albumin-chlorophyll complex model of photosynthetic protein-pigment complexes

  • L. Szalay
  • E. Tombácz
  • Z. Várkonyi
  • Á. Faludi-Dániel
Atomic and Molecular Physics

Abstract

The absorption, fluorescence, fluorescence polarization and circular dichroism spectra of an artificial complex human serum albumin-chlorophyll-a were measured at 20°C in buffer solution (pH 7.2) and the effect of detergents (digitonin, sodium lauryl sulfate, above critical micelle concentration) was studied. A strong chlorophyll-chlorophyll interaction found in the complex is removed by detergent treatment. The ionic detergent sodium lauryl sulphate induces pheophytinization and conformational changes in the protein part of the complex leading to changes in the absolute configuration of the transition moments of the pigment. Profound changes in the fluorescence polarization spectra are caused by both detergents. The effects of detergents suggest that the possibility of similar phenomena in treating plant material should be rigorously considered.

Keywords

Circular Dichroism Spectrum Sodium Lauryl Sulphate Digitonin Transition Moment Ionic Detergent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© with the authors 1982

Authors and Affiliations

  • L. Szalay
    • 1
  • E. Tombácz
    • 1
  • Z. Várkonyi
    • 1
  • Á. Faludi-Dániel
    • 2
  1. 1.Institute of BiophysicsJózsef Attila UniversitySzegedHungary
  2. 2.Institute of Plant Physiology, Biological Research CenterHungarian Academy of SciencesSzegedHungary

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