Detergent effects on an albumin-chlorophyll complex model of photosynthetic protein-pigment complexes

  • L. Szalay
  • E. Tombácz
  • Z. Várkonyi
  • Á. Faludi-Dániel
Atomic and Molecular Physics


The absorption, fluorescence, fluorescence polarization and circular dichroism spectra of an artificial complex human serum albumin-chlorophyll-a were measured at 20°C in buffer solution (pH 7.2) and the effect of detergents (digitonin, sodium lauryl sulfate, above critical micelle concentration) was studied. A strong chlorophyll-chlorophyll interaction found in the complex is removed by detergent treatment. The ionic detergent sodium lauryl sulphate induces pheophytinization and conformational changes in the protein part of the complex leading to changes in the absolute configuration of the transition moments of the pigment. Profound changes in the fluorescence polarization spectra are caused by both detergents. The effects of detergents suggest that the possibility of similar phenomena in treating plant material should be rigorously considered.


Circular Dichroism Spectrum Sodium Lauryl Sulphate Digitonin Transition Moment Ionic Detergent 
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  1. 1.
    J. P. Thornber, T. P. Markwell and S. Reinman, Photochem. Photobiol.,29, 1205, 1979.; J. Barber, ibid., Photochem. Photobiol.,30, 203, 1980.CrossRefGoogle Scholar
  2. 2.
    Proceedings of the Vth International Congress on Photosynthesis, Kallithea, Greece, Sept. 1980. Ed. G. Akoyunoglou, Balaban International Science Service, Philadelphia, 1981.Google Scholar
  3. 3.
    R. G. Hiller, in Proc. Vth International Congress on Photosynthesis, see Ref. [2].Google Scholar
  4. 4.
    J. P. Markwell, J. P. Thornber and R. T. Boggs, Proc. Natl. Acad. Sci. USA,76, 1233, 1979.CrossRefADSGoogle Scholar
  5. 5.
    Yu. E. Giller, G. B. Krasichoa and D. I. Saposhnikov, Biofizika (Moscow)15, 38, 1970; Yu. E. Giller, L. N. Yuhanova and S. K. Abdullaeva, Dokl. Akad. Nauk SSSR,207, 1475, 1972; M. Dinant and J. Aghion, Photochem. Photobiol.,17, 25, 1973; M. Widart, M. Dinant, F. Téchy and J. Aghion, Photobiochem. Photobiophys.,1, 103, 1980.Google Scholar
  6. 6.
    A. Meister and E. Brecht, Biochem. Physiol. Pflanzen,174, 305, 1979.Google Scholar
  7. 7.
    G. M. Sherman, Nature,224, 1108, 1963.CrossRefADSGoogle Scholar
  8. 8.
    E. Tombácz and E. Vozáry, Acta Biol. Univ. Szeged,26, 33, 1980.Google Scholar
  9. 9.
    E. I. Zenkevich, G. A. Kochubeev and A. M. Kulba, Biofizika (Moscow)26, 389, 1981.Google Scholar
  10. 10.
    E. Tombácz, Z. Várkonyi and L. Szalay, Zh. Prikl. Spectr. (Minsk)36, 64, 1982.Google Scholar
  11. 11.
    S. G. Boxer, 9th Ann. Meeting. Amer. Soc. Photobiol. Program and Abstract. June 14–18, p. 61. 1981.Google Scholar
  12. 12.
    Yu. E. Giller, Mol. Biol. (Moscow),14, 1057, 1980.Google Scholar
  13. 13.
    A. Wild, Z. Pflanzenphysiol.,100, 1, 1980; J. M. Anderson, FEBS Lett.,117, 327, 1980 J. H. Argyroudi-Akoyunoglou, A. Castorinis, Arch. Biochem. Biophys.,200, 326, 1980.Google Scholar
  14. 14.
    M. D. Illina, E. A. Kotova and A. Yu. Borisov, Biochim. Biophys. Acta,636, 193, 1981.CrossRefGoogle Scholar
  15. 15.
    A. C. Stewart, FEBS Lett.,114, 67, 1980.CrossRefGoogle Scholar
  16. 16.
    D. B. Knaff, R. Malkin, J. C. Myron and M. Stoller, Biochim. Biophys. Acta,459, 402, 1977; T. Szitó, J. Hevesi and E. Bálint, Zh. Prikl. Spectr. (Minsk)33, 751, 1980; E. Lehoczki and K. Csatorday, Biochim. Biophys. Acta,396, 86, 1975; E. Lehoczki, ibid. Biochim. Biophys. Acta,408, 223, 1975.CrossRefGoogle Scholar
  17. 17.
    E. Vozáry and L. Szalay, Acta Phys. Chem. Szeged,23, 17, 1976.Google Scholar
  18. 18.
    A. Helenius and K. Simons, Biochim, Biophys. Acta,415, 29, 1975.Google Scholar
  19. 19.
    L. Szalay, Z. Várkonyi and E. Tombácz, Biofizika (Moscow),26, 628, 1981.Google Scholar
  20. 20.
    P. Koka and S. Pill-Soon, Biochim. Biophys. Acta,495, 220, 1977.Google Scholar
  21. 21.
    I. N. Stadnichuk and M. V. Guseva, Photosynthetica,15, 221, 1981.Google Scholar
  22. 22.
    G. P. Gurinovich, A. N. Sevchenko and K. N. Solovjev, Spectroscopy of Chlorophylls and Related Compounds. Translated from Russian AEC-tr-7199, Chemistry (TID-4500) US Atomic Energy Commission, Div. Techn. Inform. p. 241, 1971.Google Scholar
  23. 23.
    D. Frackowiak and Z. Kojro, Proc. Int. Conf. Luminescence, Budapest, Akadémiai Kiadó, 301, 1966.Google Scholar
  24. 24.
    R. K. Clayton, Photosynthesis: Physical Mechanisms and Chemical Patterns. IUPAB Biophysics Series; 4. Cambridge University Press, p. 147, 1980.Google Scholar

Copyright information

© with the authors 1982

Authors and Affiliations

  • L. Szalay
    • 1
  • E. Tombácz
    • 1
  • Z. Várkonyi
    • 1
  • Á. Faludi-Dániel
    • 2
  1. 1.Institute of BiophysicsJózsef Attila UniversitySzegedHungary
  2. 2.Institute of Plant Physiology, Biological Research CenterHungarian Academy of SciencesSzegedHungary

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