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Raman study of isolated and “in situ” T7 phage DNA: conformation and possible interaction with the proteins

  • D. Aslanian
  • Gy Rontó
  • K. Tóth
Atomic and Molecular Physics

Abstract

The Raman spectrum of T7 phage is shown to be dominated by the vibrations of the constituent DNA (backbone chain and bases). These vibrations are compared with their counterparts in the spectrum of isolated T7 DNA. Inspite of the great similarity between the two spectra there are some differences which suggest that the bases play an important role in the DNA-protein interaction. The sites of these interactions appear to involve the CH3 and C=O groups of thymine, C=O groups of cytosine and N7—C8 group of guanine.

Some preliminary observations are given on the UV induced changes in isolated DNA.

Keywords

Raman Spectrum Cytosine Thymine Deoxyribose Backbone Chain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Gy. Rontó, M. M. Agamalyan, G. M. Drabkin, L. A. Feigin and Yu. A. Lvov (to be published in Biophys. J.)Google Scholar
  2. 2.
    P. Camerini-Otrero et al., Biochemistry,13, 960, 1974.CrossRefGoogle Scholar
  3. 3.
    J. A. Harpst, Biophys. Chem.,11, 295, 1980.CrossRefGoogle Scholar
  4. 4.
    Gy. Rontó, Theses for D.S.C. Budapest, 1979.Google Scholar
  5. 5.
    A. Fekete and Gy. Rontó, Studia biophys.,80, 165, 1980.Google Scholar
  6. 6.
    K. Tóth, Theses, Université P. et. M. Curie, Paris, 1981.Google Scholar
  7. 7.
    S. Dobos, J. J. Fidy and Zs. Laczkó, J. Mol. Structure,61, 251 1980.CrossRefADSGoogle Scholar
  8. 8.
    W. L. Peticolas, in Infrared and Raman Spectroscopy of Biological Molecules (Theo M. Theophanides ed.) NATO Advanced Institutes Series, D. Reidel Publishing Company, Doidrecht, Holland, 153–165, 1979.Google Scholar
  9. 9.
    M. Tsuboi, Proceedings of 5th Intern. Confer. on Raman Spectroscopy, Freiburg, 1976 (E. D. Schmidt et al. ed.) Hans Ferdinand Schulz Verlag. D-7800 Freiburg im Breisgau, 135—143, 1976.Google Scholar
  10. 10.
    K. A. Hartman, N. Clayton and G. J. Thomas Jr. Biochem. Biophys. Research Communic.,50, 942, 1973.CrossRefGoogle Scholar
  11. 11.
    G.J. Thomas Jr. and P. Murphy, Science,188, 1205, 1975.CrossRefADSGoogle Scholar
  12. 12.
    G. J. Thomas Jr., B. Prescott, P. E. McDonald-Ordzie and K. A. Hartman, J. Mol. Biol.,102, 103, 1976.CrossRefGoogle Scholar
  13. 13.
    T. T. Herskovits and J. Brahms, Biopolymers,15, 687, 1976.CrossRefGoogle Scholar
  14. 14.
    J. Liquier, J. Taboury, E. Taillandier and J. Brahms, Biochemistry,16, 3262, 1977.CrossRefGoogle Scholar
  15. 15.
    S. Gáspár, Gy Rontó and G. Müller, Teitsch. Allg. Microbiol.,19, 163, 1979.CrossRefGoogle Scholar
  16. 16.
    J. M. Strauss and R. L. Sinsheimer, J. Mol. Biol.,7, 43, 1963.CrossRefGoogle Scholar
  17. 17.
    I. Mandell and A. D. Hershey, Anal. Biochem.,1, 66, 1960.CrossRefGoogle Scholar
  18. 18.
    S. C. Erfurth, E. J. Kiser and W. L. Peticolas, Proc. Natl. Acad-Sci. USA,69, 938, 1972.CrossRefADSGoogle Scholar
  19. 19.
    D. C. Goodwin and J. Brahms, Nucleic. Acids. Research,5, 835, 1978.CrossRefGoogle Scholar
  20. 20.
    R. C. Lord and G. J. Thomas, Spectrochim. Acta,23A, 2551, 1967.Google Scholar
  21. 21.
    S. C. Erfurth, P. J. Bond and W. L. Peticolas, Biopolymers,14, 1245, 1975.CrossRefGoogle Scholar
  22. 22.
    Yu. A. Rolbin, D. I. Svergun, L. A. Feigin, S. Gáspár and Gy. Rontó, Dokl. Akad. Nauk,255, 1497, 1980.Google Scholar
  23. 23.
    R. Herbeck, T. J. Yu and W. L. Peticolas, Biochemistry,15, 2656, 1976.CrossRefGoogle Scholar
  24. 24.
    J. C. Girod, W. C. Johnson Jr., S. K. Huntington and M. F. Maestre, Biochemistry,12, 5092, 1973.CrossRefGoogle Scholar
  25. 25.
    L. Chinsky, P. Y. Turpin, M. Duquesne and J. Brahms, Biopolymers,17, 1347, 1978.CrossRefGoogle Scholar
  26. 26.
    M. Tsuboi, J. Raman Spectroscopy,2, 609, 1974.CrossRefADSGoogle Scholar

Copyright information

© with the authors 1982

Authors and Affiliations

  • D. Aslanian
    • 1
  • Gy Rontó
    • 1
  • K. Tóth
    • 2
  1. 1.Laboratoire de Physique des Solides et Spectroscopie des SolidesAssocié au CNRS la 154 Université P. ET M. CurieParis Cedex 05France
  2. 2.Institute of BiophysicsSemmelweis Medical UniversityBudapestHungary

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