Proceedings: Plant Sciences

, Volume 100, Issue 4, pp 225–231 | Cite as

Light activation of enzymes in relation to leaf age inVigna unguiculata (L.) Walp andZea mays L.

  • M Noorunisa Begam
  • M Vivekanandan


The effects of light-dark transition and aplastidic condition by the photobleaching herbicide, 3-amino-1,2,4-triazole, on the activities of enzymes likein vivo nitrate reductase, peroxidase, polyphenol oxidase and catalase were studied in leaves of different ages of 30-day old plants ofVigna unguiculata andZea mays. The activity of nitrate reductase was found to be higher in young leaves and showed a gradual decline at the time of maturity and senescence. In amitrole-bleached young leaves,in vivo nitrate reductase activity was significantly reduced. Although peroxidase is a light-activated enzyme, the enzyme was more active only in fully mature and senescing leaves ofVigna unguiculata. InZea mays, peroxidase activity was more in mature leaves compared to senescent leaves. Interesting feature of this enzyme was that its activity increased upon dark treatment inVigna unguiculata. A similar trend was also observed in polyphenol oxidase activity in mature and senescent leaves ofVigna unguiculata andZea mays. Foliar spray of amitrole increased peroxidase and polyphenol oxidase activities in the young leaves ofZea mays andVigna unquiculata. The results are discussed in relation to age of leaves and the presence or absence of leaf plastids.


Nitrate reductase peroxidase catalase Zea mays polyphenol oxidase amitrole Vigna unguiculata 


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Copyright information

© Indian Academy of Sciences 1990

Authors and Affiliations

  • M Noorunisa Begam
    • 1
  • M Vivekanandan
    • 1
  1. 1.School of Life SciencesBharathidasan UniversityTiruchirapalliIndia

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