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Transmission of seed-borne inoculum ofMacrophomina phaseolina from seed to plant

  • Tribhuwan Singh
  • Dalbir Singh
Article
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Abstract

Macrophomina phaseolina is a serious pathogen which is externally as well as internally seed-borne. It causes failure of seed germination and browning and rotting of seedlings. The presence of pathogen in infected and healthy looking seedlings was tested by clearing, sectioning and incubation techniques. After 8 weeks almost every surviving plant developed pale yellow to brown circular or oval concentric spots on leaves, stem and capsules. Mycelium and microsclerotia were observed in the peripheral region of the lesions. Splitted root, stem and capsule also showed the presence of microsclerotia. Cleared wholemounts of leaf and stem and T.S. and L.S. of stem showed inter- and intracellular mycelium in cortex, xylem and pith cells. Microsclerotia were also observed. In capsule, infection was recorded on its inner wall, septum, placenta and seeds spreading from base to apex.

Keywords

Macrophomina phaseolina seed-borne transmission Sesamum indicum 

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Copyright information

© Indian Academy of Sciences 1982

Authors and Affiliations

  • Tribhuwan Singh
    • 1
  • Dalbir Singh
    • 1
  1. 1.Department of BotanyUniversity of RajasthanJaipurIndia

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