Transmission of seed-borne inoculum ofMacrophomina phaseolina from seed to plant

  • Tribhuwan Singh
  • Dalbir Singh


Macrophomina phaseolina is a serious pathogen which is externally as well as internally seed-borne. It causes failure of seed germination and browning and rotting of seedlings. The presence of pathogen in infected and healthy looking seedlings was tested by clearing, sectioning and incubation techniques. After 8 weeks almost every surviving plant developed pale yellow to brown circular or oval concentric spots on leaves, stem and capsules. Mycelium and microsclerotia were observed in the peripheral region of the lesions. Splitted root, stem and capsule also showed the presence of microsclerotia. Cleared wholemounts of leaf and stem and T.S. and L.S. of stem showed inter- and intracellular mycelium in cortex, xylem and pith cells. Microsclerotia were also observed. In capsule, infection was recorded on its inner wall, septum, placenta and seeds spreading from base to apex.


Macrophomina phaseolina seed-borne transmission Sesamum indicum 


  1. Chidambaram P and Mathur S B 1975 Production of pycnidia byMacrophomina phaseolina;Trans. Br. Mycol. Soc. 64 165–167CrossRefGoogle Scholar
  2. Cougnée M 1963 Études sur L’ anthraenase des Cotoniers;G. Barbadense;Cotton. et Fibres Trop. 18 149–150Google Scholar
  3. Fakir Golam A, Rao M H and Thirumalachar M J 1976 Seed transmission ofMacrophomina phaseolina in sunflower;Plant Dis. Rep. 44 221Google Scholar
  4. Gemawat P D and Verma O P 1974 Root and stem rot ofSesamum in Rajasthan. Evaluation of varieties (Macrophomina phaseolina) Indian. J. Mycol. Plant Pathol. 4 76–77Google Scholar
  5. Jain A C and Kulkarni S N 1965 Root and stem rot ofSesamum;Indian Oil Seeds J. 9 201–203Google Scholar
  6. McRae W 1930 Report of the Imperial Mycologist Scient;Rep. Agric. Res. Inst. Pusa 1928–29 51–56Google Scholar
  7. Mehta P R 1951 Observations on new and known diseases of crop plants of Uttar Pradesh;Plant Prot. Bull. New Delhi 3 7–12Google Scholar
  8. Meiri Aliza and Solel Z 1963 Transmission of charcoal rot by sesame seeds;Phytopathol. Mediterr. 2 90–92Google Scholar
  9. Mishra R P, Singh B P and Joshi L K 1973 Pod susceptibility of different varieties of till (Sesamum orientale) toMacrophomina phaseoli (Maubl.) Ashby;J.N.K.V.V. Res. J. 7 288–289Google Scholar
  10. Noble Mary, De Tempe J and Neergaard P 1958 An annotated list of seed-borne diseases;C.M.I. Kew 159Google Scholar
  11. Richardson M J 1979 Annotated list of seed-borne diseases; 3rd edition,Proc. Int. Seed Test. Ass. 23 1–320Google Scholar
  12. Parasar R D and Suryanarayana D 1971 Polygalecturonase activity ofMacrophomina phaseolina the incitant root rot ofSesanum indicums;Indian Phytopath. 24 559–562Google Scholar
  13. Pearl R J 1923 Report of the mycologist to the Govt. of the Central Provinces and Berar. Rept. Deptt. Agric. Central Provinces and Berar for the year ending 30th June 1922 19–20Google Scholar
  14. Singh T and Singh D 1979 Anatomy of penetration ofMacrophomina phaseolina in seeds of sesame;Recent researches in plant sciences (ed.) S S Bir Ludhiana: Kalyani Publisher) pp. 603–606Google Scholar
  15. Sundararaman S 1931 Administration report of the mycologist for the year 1929–30;Dept. Agric. Madras Google Scholar
  16. Sundararaman S 1932 Administration report of the mycologist for the year 1930–31;Dept. Agric. Madras Google Scholar
  17. Verma O P and Daftari L N 1974 Amount of seed-borne inoculum ofMacrophomina phaseolina and its effect on mortality and growth ofSesamum seedlings;Indian Phytopath. 27 130–131Google Scholar

Copyright information

© Indian Academy of Sciences 1982

Authors and Affiliations

  • Tribhuwan Singh
    • 1
  • Dalbir Singh
    • 1
  1. 1.Department of BotanyUniversity of RajasthanJaipurIndia

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