Xylanolytic activity of thermophilicSporotrichum sp. andMyceliophthora thermophilum

  • A K Dubey
  • Bhavdish N Johri


Extracellular xylanase of 5 isolates of thermophilicSporotrichum sp. andMyceliophthora thermophilum was studied in media containing wheat straw, wheat bran, paddy straw, paddy husk, sugar cane bagasse and local grass (Cyanodon dactylon L). Maximum D-xylanase (0·92 IU/ml) was secreted on 6th day bySporotrichum sp. strain 1 in a basal medium that contained 5% (w/v) wheat straw; treatment of straw by 4% NaOH prior to fungal fermentation did not alter xylanase yields appreciably. Temperature and pH optima for enzyme production were 45°C and 5·0 respectively. Enzyme activity showed a temperature optima of 70°C, pH maxima of 5·5 and a substrate level of 3% larch wood xylan. Xylanase ofSporotrichum sp. strain 1 was inducible and highly thermostable; nearly 70% of activity was retained even after 5 min exposure at 80°C. Associated cellulolytic activity (0·12 IU/ml) was detected in the crude enzyme preparation.


Xylanase thermophilic lignocellulose fungus 


  1. Bisaria R, Madan M and Bisaria V S 1987 Biological efficiency and nutritive value ofPleurotus sajor-caju cultivated on different agroresidues;Biol. Wastes (UK) 19 239–255CrossRefGoogle Scholar
  2. Carrol G and Petrini O 1983 Patterns of substrate utilization by some fungal endophytes from coniferous foliage;Mycologia 75 53–63CrossRefGoogle Scholar
  3. Chouhan S C 1985Extracellular proteases of thermophilic fungi, Ph.D. Thesis, Bhopal University, BhopalGoogle Scholar
  4. Clarke R T J, Bailey R W and Cailard B D E 1969 Growth of rumen bacteria on plant cell wall polysaccharides;J. Gen. Microbiol. 65 79–86Google Scholar
  5. Dekker R H D 1983 Bioconversion of hemicellulose: Aspects of hemicellulase production byTrichoderma reesei QM 9414 and enzymatic saccharification of hemicellulose;Biotechnol. Bioeng. 25 1127–1146PubMedCrossRefGoogle Scholar
  6. Dekker R F H and Richard G N 1976 Hemicellulases: their occurrence, purification, properties and mode of action;Adv. Carbohy. Chem. Biochem. 32 277–352CrossRefGoogle Scholar
  7. Flannigan B and Sellars P N 1972 Activities of thermophilous fungi from barley kernels against arabinoxylan and carboxymethyl cellulose;Trans. Br. Mycol. Soc. 58 338–341Google Scholar
  8. Flannigan B and Sellars P N 1977 Amylase, β-glucosi lase and β-xylosidase activity of thermotolerant and thermophilic fungi isolated from barley;Trans. B Mycol. Soc. 69 316–317CrossRefGoogle Scholar
  9. Lowry O H, Rosebrough N J, Farr A L and Randall R J 1951 Protein measurement with the Folin Phenol Reagent;J. Biol. Chem. 193 265–275PubMedGoogle Scholar
  10. Maheshwari R and Kamlam P T 1985 Isolation and culture of thermophilic fungus,Melanocarpus albomyces and factors influencing the production and activity of xylanase;J. Gen. Microbiol. 131 3017–3027Google Scholar
  11. Miller G L 1959 Use of dinitrosalicylic acid reagent for determination of reducing sugars;Anal. Chem. 31 426–428CrossRefGoogle Scholar
  12. Saddler J N, Mesttartree M, Levitin N and Briwnell N H 1983 Utilization of enzymatically hydrolyzed wood hemicelluloses by microorganisms for production of liquid fuels;Appl. Environ. Microbiol. 45 153–160PubMedGoogle Scholar
  13. Satyanarayana T and Johri B N 1983 Variation in xylanolytic activity of thermophilic fungi;Bionature 3 39–41Google Scholar
  14. Singh P 1982Thermophilous microfungi of soil and air and their enzymatic activity, Ph.D. Thesis, Bhopal University, BhopalGoogle Scholar
  15. Thaysen A C and Bunker H J 1927 The microbiology of cellulose, hemicellulose, pectin and gums (London: Oxford University Press)Google Scholar
  16. Yoshioka H, Chavanich S, Nilubol N and Hayashida S 1981 Production and characterization of thermostable xylanase fromTalaromyces bayssochlamydoides YH-150;Agric. Biol. Chem. 45 579–586Google Scholar

Copyright information

© Indian Academy of Sciences 1987

Authors and Affiliations

  • A K Dubey
    • 1
  • Bhavdish N Johri
    • 1
  1. 1.Department of MicrobiologyGB Pant University of Agriculture and TechnologyPantnagarIndia

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