Proceedings: Plant Sciences

, Volume 94, Issue 4–6, pp 567–574 | Cite as

Survey of some Indian soils for laccase producing fungi and their lignin degrading ability

  • D S Arora
  • D K Sandhu


One hundred and fifty soil samples collected from three climatologically different sites were screened for laccase production using lignin-guaiacol agar plates. All the 12 fungi isolated excepting one gave positive test for qualitative lignin degradation. Five fungi were selected further for quantitative study of wood/lignin degradation. The basidiomycetes proved to be better laccase producers and decomposers of wood/lignin. These gave comparable results to a known white-rot fungus,Polyporus versicolor (L.) Fr. and degraded wood sawdust causing a total weight loss of 7–8% and lignin loss up to 10% in 30 days.


Laccase lignin wood soils 


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  1. Ander P and Eriksson K E 1976 The importance of phenoloxidase activity in lignin degradation by the whiterot fungusSporotrichum pulverulentum;Arch. Microbiol. 109 1–8CrossRefGoogle Scholar
  2. Ander P and Eriksson K E 1978 Lignin degradation and utilization by microorganisms;Prog. Indian Microbiol. 14 1–58Google Scholar
  3. Barron G L 1972The genera of hyphomycetes from soil (New York: Robert E Krieger Publishing Company)Google Scholar
  4. Bavendamm W 1928 Uber des vorkommen und den Nachweis von oxydasin bei Holzzerstorenden Pilzen;Ze. Pflanzenkrankheiten und Pflanzenschutz. 38 257–276Google Scholar
  5. Boidin J 1951 Recherche de la tyrosinase et de la laccase chez les Basidiomycetes en culturepure. Milieuse differentiels. Interret Systematique;Rev. Mycol. 16 173–197Google Scholar
  6. Davidson R W, Campbell W A and Blaisdell D J 1938 Differentiation of wood decaying fungi by their reactions on gallic and tannic acid medium;J. Agric. Res. (USA) 57 683–695Google Scholar
  7. Effland M J 1977 Modified procedure to determine acid insoluble lignin in wood and pulp;Tappi 60 143–144Google Scholar
  8. Ellis M B 1971 Dematiaceous hyphomycetes. Commonwealth Mycological Institute, Kew, Surrey, EnglandGoogle Scholar
  9. Ellis M B 1976 More dematiaceous hyphomycetes. Commonwealth Mycological Institute, Kew, Surrey, EnglandGoogle Scholar
  10. Eslyn W E, Kirk T K and Effland M J 1975 Changes in the chemical composition of wood caused by six softrot fungi;Phytopathology 65 473–476CrossRefGoogle Scholar
  11. Fahraeus G 1949 On the oxidation of phenolic compounds by woodrotting fungi;Ann. R. Agric. Coll. Sweden 16 618–629Google Scholar
  12. Fahraeus G, Tullander V and Ljungren H 1958 Production of high laccase yields in cultures of fungi;Physiol. Plant 11 631–643CrossRefGoogle Scholar
  13. Haider K and Trojanowski J 1981 A comparison of the degradation of14C-labelledDHP and corn stalk lignins by micro- and macro-fungi and by bacteria inLignin biodegradation: Microbiology, chemistry and potential applications (eds) T K Kirk, T Higuchi and H Chang (Boca Raton, Florida: CRC Press) Vol 1 pp. 111–134Google Scholar
  14. Harkin J M and Obst J R 1973 Syringaldazine an effective reagent for detecting laccase and peroxidase in fungi;Experientia 29 381–387CrossRefGoogle Scholar
  15. Higuchi T 1982 Biodegradation of lignin: Biochemistry and potential applications;Experientia 38 159–166CrossRefGoogle Scholar
  16. Kirk T K and Chang H M 1975 Decomposition of lignin by white-rot fungi. II. Characterization of heavily degraded lignins from decayed spruce;Holzforschung 29 56–64CrossRefGoogle Scholar
  17. Kirk T K and Harkin J M 1973 Lignin biodegradation and the bioconversion of wood. Forest products and the environment;Alche. Symp. Ser. 69 124–134Google Scholar
  18. Kirk T K and Kelman A 1965 Lignin degradation as related to the phenoloxidases of selected wood decaying basidiomycetes;Phytopathology 55 739–745Google Scholar
  19. Lindeberg G 1948 On the occurrence of polyphenoloxidases in soil inhabiting basidiomycetes;Physiol. Plant 1 196–205CrossRefGoogle Scholar
  20. Nobles M K 1958 A rapid test for extracellular oxidase in cultures of wood inhabiting hymenomycetes;Can. J. Bot. 36 91–99CrossRefGoogle Scholar
  21. Norris D M 1980 Degradation of14C-labelled lignins and14C-labelled aromatic acids byFusarium solani;Appl. Environ. Microbiol. 40 376–380PubMedGoogle Scholar
  22. Rosenberg S L 1980 Patterns of diffusibility of lignin and carbohydrate degrading systems in wood-rotting fungi;Mycologia 72 798–812CrossRefGoogle Scholar
  23. Sandhu D K and Arora D S 1984 Laccase production byPolyporus versicolor on different substrates;Acta Biotechnol. 4 49–57CrossRefGoogle Scholar
  24. Sundman V and Nase L 1971 A simple plate test for direct visualization of biological lignin degradation;Pap. Puu. 53 67–71Google Scholar
  25. Westermark U and Eriksson K E 1974 Carbohydrate dependent enzymic quinone reduction during lignin degradation;Acta Chem. Scand. B28 204–208CrossRefGoogle Scholar
  26. Zadrazil F 1977 The conversion of straw into feed by basidiomycetes;Eur. J. Appl. Microbiol. Biotechnol. 4 273–281Google Scholar
  27. Zadrazil F 1980 Conversion of different plant waste into feed by basidiomycetes;Eur. J. Appl. Microbiol. Biotechnol. 9 243–248CrossRefGoogle Scholar

Copyright information

© Indian Academy of Sciences 1985

Authors and Affiliations

  • D S Arora
    • 1
  • D K Sandhu
    • 1
  1. 1.Department of BiologyGuru Nanak Dev UniversityAmritsarIndia

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