The autoxidation of thiol aminoacids and ascorbate and their cooperative effects as antioxidants with trolox in micelles and lipid bilayers

  • L. R. C. Barclay
  • K. A. Dakin
  • J. A. Y. Khor


The thiols cysteine (1), homocysteine (2), acetylcysteine (3), glutathione (4), and dithioerythritol (5) underwent autoxidation with controlled rates of chain initiation (Ri) when driven by azobis (2-amidino propane hydrochloride (ABAP). Cysteine exhibits the largest rate constant. Thils2 and4 inhibited the facile self-initiated autoxidation of ascorbate and regenerated ascorbate from its oxidation product, dehydroascorbic acid. Thiols1 and2 inhibited ABAP- initiated peroxidation of dilinoleoyl phosphatidylcholine (DLPC) membranes with a kinh similar to that of 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylate (Trolox). Thiols14 all acted cooperatively with Trolox to inhibit ABAP-initiated peroxidation of DLPC membranes. Stoichiometric factors, n=0.31−0.63, are attributed to oxidative wasting reactions.

Each thiol,2, 4, and5 when combined with ascorbate further extended the inhibition period mediated by Trolox during peroxidation of linoleate initiated by lipid-soluble di-tert-butylhyponitrite (DBHN) in sodium dodecly sulfate (SDS) micelles. The spin trap phenyl-tert-butylnitrone (PBN) exhibited only retardant (not antioxidant) activity during peroxidation of linoleate initiated by DBHN or ABAP in SDS micelles.


Thiol Homocysteine Linoleate Induction Period Trolox 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer 1995

Authors and Affiliations

  • L. R. C. Barclay
    • 1
  • K. A. Dakin
    • 1
  • J. A. Y. Khor
    • 1
  1. 1.Chemistry DepartmentMount Allison UniversitySackvilleCanada

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