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Research on Chemical Intermediates

, Volume 21, Issue 3–5, pp 313–328 | Cite as

Homo- and hetero-nuclear 2D NMR techniques: Umambiguous structural probes for non-cyclic benzyl aryl ethers in soluble lignin samples

  • R. M. Ede
  • I. Kilpeläinen
Article

Abstract

A number of two-dimensional NMR experiments are evaluated with respect to their ability to provide unambiguous evidence for the presence or absence of non-cyclic benzyl aryl ether (α-O-4) structures in soluble lignin samples. The most suitable techniques, in terms of both sensitivity and structural information content were the homonuclear Hartmann-Hahn (HOHAHA) and heteronuclear multiple quantum coherence (HMQC) techniques. By spiking an acetylated Pinus radiata MWL sample with an oligomeric α-O-4 ether model compound, it was possible to determine a detection limit of <0.3 structures per 100 lignin C9 units for both the HOHAHA and HMQC techniques. From this, and other work, it can be shown that if α-O-4 structures are present in MWL samples, they are present at a level below the detection limit of these experiments. The implications of these results are discussed in terms of lignin biosynthesis and reactivity.

Keywords

Lignin Soluble Lignin Milled Wood Lignin Heteronuclear Multiple Quantum Coherence Interunit Linkage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer 1995

Authors and Affiliations

  • R. M. Ede
    • 1
  • I. Kilpeläinen
    • 2
  1. 1.Department of ChemistryThe University of WaikatoHamiltonNew Zealand
  2. 2.Institute of BiotechnologyFIN-00014 University of HelsinkiFinland

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