Absence of secretion of acetylcholine on stimulation of nerves in an unstriated muscle

  • Inderjit Singh
  • Shakuntala Sharma
  • O. P. Bhatnagar


1. Frog’s stomach muscle when immersed in eserinised Ringer’s solution for 2 hours shows no leakage of acetylcholine, whether extended or unextended.

2. Electrical stimulation of the muscle or its nerves does not release any acetylcholine.

3. Depolarisation with strong solutions of potassium chloride does not liberate acetylcholine.

4. Immersion in strong solutions of calcium chloride does not liberate acetylcholine.

5. Heating to 50–60° C., or treatment with ether liberates acetylcholine; the muscle also relaxes actively if heated. Adrenaline or noradrenaline is also liberated by heating.

6. As heating to 50–60° C. causes active relaxation as well as liberation of acetylcholine, it is suggested that acetylcholine is in some way connected with contractility by direct action on the contractile mechanism.

7. The above results were obtained in autumn, but in winter acetylecholine was liberated.


Acetylcholine Calcium Chloride Strong Solution Potassium Chloride Neuromuscular Transmission 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Burns, J. H. ..Functions of Autonomic Transmitters, 1950, 20.Google Scholar
  2. Chujyo..Amer. J. Physiol., 1952,170, 668.PubMedGoogle Scholar
  3. Feldberg, W...J. Physiol., 1948,107, 372.PubMedGoogle Scholar
  4. Jullien, A., Repplinger, J. and Cardot, J.C.R. Soc. Biol., Paris, 1954,148, 1258. Quoted from Burns, J. H.Google Scholar
  5. Nachmonsohn, D... Fulton’sText-Book of Physiology, 17th Edition, W. B. Saunders & Co., London, 1955, 192.Google Scholar
  6. Richter, D. and Crossland, J...Am. J. Physiol., 1949,159, 247.PubMedGoogle Scholar
  7. Singh, I...Proc. Ind. Acad. Sci., 1958,48, 131.Google Scholar
  8. Singh, S. I. and Singh..Proc. Ind. Acad. Sci., 1954,40, 125.Google Scholar
  9. —————..Nature, 1950,166, 647.PubMedCrossRefGoogle Scholar
  10. Singh, I. and Bhatt, J. V...Proc. Ind. Acad. Sci., 1957,45, 64.Google Scholar
  11. —— and Acharya, A. K... Ibid., 1957a,46, 285.Google Scholar
  12. —————..Ind. J. Physiol. and Pharmacol., 1957b,1, 265.Google Scholar
  13. —————.. Ibid., 1958a,2, 262.Google Scholar
  14. —————..Proc. Ind. Acad. Sci., 1958b,48, 41.Google Scholar

Copyright information

© Indian Academy of Sciences 1959

Authors and Affiliations

  • Inderjit Singh
    • 1
  • Shakuntala Sharma
    • 1
  • O. P. Bhatnagar
    • 1
  1. 1.From the Department of PhysiologyMedical CollegeAgra

Personalised recommendations