Effectiveness and efficiency of single and combined treatments of radiations and ethyl methane sulphonate inSorghum
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The effect of single treatments with X- and gamma rays and EMS and combination treatments of gamma rays sand EMS on survival, seedling growth and seed fertility in M1 and chlorophyll mutation frequency in M2 was studied in the variety Co. 18 ofS. subglabrascens. There was an increase in the reduction of survival and seedling height and seed fertility with an increase in the dose of mutagens. Combined treatments were found to be more effective in reducing the survival and height of seedlings than single treatments, while EMS caused more drastic effect on seed fertility than rest of the mutagens. The frequency of chlorophyll mutations in the M2 increased with dose upto a certain dose level and then levelled off at high doses. EMS induced higher frequency of chlorophyll mutations than treatments with radiations. Combination treatments were found to be the most efficient in inducing the chlorophyll mutations as compared to single treatments. Mutagenic effectiveness was found to be the maximum at high doses of gamma rays and EMS, whereas higher efficiency values were observed at lower concentrations of EMS and middle doses of radiations. EMS was found to be the most effective and efficient mutagen than rest of the mutagens.
KeywordsCombine Treatment Single Treatment Drastic Effect Seedling Height Chemical Mutagen
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