Studies in pectic enzymes of parasitic fungi
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Highly active protopectinase and polygalacturonase (PG) enzymes were secreted byPenicillium expansum Link. on 2·5% decoction of lucerne, pectin asparagin and modified Czapek’s media. Ammonium nitrate was found to be a better nitrogen source for the secretion of pectic enzymes than asparagin or sodium nitrate. Best secretion of protopectinase was in cultures after 4 days incubation at 20° C.
Pectin-esterase, when tested at 6·2 pH, was found to be of negligible activity in 4 days old cultures of the fungus.
Protopectinase and polygalacturonase (PG) were found to be adaptive enzymes which were not produced in absence of pectic substances.
KeywordsPectin Ammonium Nitrate Sodium Nitrate Potassium Dihydrogen Phosphate Parasitic Fungus
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