Differential fluorescence of the chromocenters and nucleolar equivalents of the yeast nucleus in acridine orange

  • Saraswathy Royan
  • M. K. Subramaniam


The nucleus with its limiting membrane and organelles was visible in the majority of the yeast cells stained vitally with the fluorochrome, acridine orange, at a dilution of 1 in 40,000. The intra-nuclear structures could be distinguished by their differential fluorescence. The chromocenters were green while the nucleolar equivalents were orange. The vacuole showed no fluorescence.


Acridine Orange Olar Equivalent Reddish Orange Bright Orange Orange Fluorescence 
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Copyright information

© Indian Academy of Sciences 1960

Authors and Affiliations

  • Saraswathy Royan
    • 1
  • M. K. Subramaniam
    • 1
  1. 1.Cytogenetics Laboratory, Department of BiochemistryIndian Institute of ScienceBangalore-12

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