Summary
A procedure for determining the nature of the products of proteolysis making use of the factor, total N/amino N, at various stages of the enzyme action, is described.
The procedure has been applied to the results of casein hydrolysis. It has been shown that under the action of crystalline trypsin, peptides with a complexity factor of 8, are chipped off from the casein. With crude trypsin, on the other hand, no such regularity is observed.
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References
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Bhagvat, K.Jour. Indian Inst. Sci., 1936,19 A, 67.
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Our thanks are due to Mr. M. Sreenivasaya for many helpful suggestions.
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Ranganathan, V., Sastri, B.N. Procedure for determining the nature of the degradation products during proteolysis. Proc. Indian Acad. Sci. 8, 243–248 (1938). https://doi.org/10.1007/BF03048213
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DOI: https://doi.org/10.1007/BF03048213