Netherlands Journal of Plant Pathology

, Volume 83, Supplement 1, pp 317–329 | Cite as

Pathogenicity of fungi isolated from stems and bulbs of lilies and their sensitivity to benomyl

  • G. J. Bollen
Use of Systemic Fungicides in Practice


In 1971 and 1972 crops of Lilium speciosum and the hybrid L. cv. ‘Enchantment’ grown from benomyl-treated planting stock suffered locally from wilting and defoliation. The symptoms were associated with lesions on the hypogeous part of the stem. From the lesions benomyl-resistant isolates of Cylindrocarpon sp. and a basidiomycete, Sistotrema sp., were obtained and also benomyl-sensitive isolates of Fusarium oxysporum f.sp. lilii. Inoculation experiments revealed that Cylindrocarpon sp. and F. oxysporum were highly pathogenic to scales buried in soil and used for propagation, as well as to young lily plants grown in vitro. In these experiments Sistotrema sp. proved to be weakly pathogenic. In greenhouse trials its pathogenicity to plants grown from bulbs planted in infested soil was not confirmed.

Since 1972 benomyl has only occasionally been used by Dutch lily growers and then only in combination with other fungicides, especially with captafol. From 1972 to 1975 resistance in the population of Cylindrocarpon sp. has gradually decreased, but has not disappeared entirely.


Fusarium Potato Dextrose Agar Mycelial Growth Fusarium Oxysporum Benomyl 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bald, J.G. & Solberg, R.A., 1960. Antagonism and synergism among organisms associated with scale tip rot of lilies. Phytopathology 50: 615–620.Google Scholar
  2. Bollen, G.J., 1971. Resistance to benomyl and some chemically related compounds in strains of Penicillium species. Neth. J. Pl. Path. 77: 187–193.CrossRefGoogle Scholar
  3. Boontjes, J., 1970. Bestrijding van schubrot en bolrot bij lelies. Praktijkmeded. Lab. voor Bloembollenonderzoek 32, 8 pp.Google Scholar
  4. Booth, C., 1966. The genus Cylindrocarpon. Mycol. Pap. 104: 56 pp.Google Scholar
  5. Dekker, J., 1977. The fungicide-resistance problem. Neth. J. Pl. Path. 83S: 159–167.Google Scholar
  6. Duineveld, T.L.J. & Beijersbergen, J.C.M., 1977. In vitro study on the benomyl tolerance of Fusarium oxysporum f. sp. tulipae. Neth. J. Pl. Path. 83S: 207–213.CrossRefGoogle Scholar
  7. Fehrmann, H., 1976. Systemische Fungizide — ein Überblick. II. Fungizid-resistenz phytopathogener Pilze. Phytopath. Z. 86: 144–185.CrossRefGoogle Scholar
  8. Geeson, J.D., 1976. Comparative studies of methyl-benzimidazol-2-yl carbamate-tolerant and sensitive isolates of Botrytis cinerea and other fungi. Trans. Br. mycol. Soc. 66: 123–129.Google Scholar
  9. Hawker, L.E. & Singh, B., 1943. A disease of lilies caused by Fusarium bulbigenum Cooke et Mass. Trans Br. mycol. Soc. 26: 116–126.CrossRefGoogle Scholar
  10. Hell, W.F. van, 1931. Onderzoekingen over ziekten van lelies. Thesis, University of Utrecht, 166 pp.Google Scholar
  11. Hoeven, E.P. van der & Bollen, G.J., 1972. The effect of benomyl on antagonism towards fungi causing foot rot in rye. Acta bot. neerl. 21: 107–108.Google Scholar
  12. Imle, E.P., 1942. Bulb rot diseases of lilies. Am. Lily Yb., Washington: 30–41.Google Scholar
  13. Kuramoto, T., 1976. Resistance to benomyl and thiophanate-methyl in strains of Penicillium digitatum and P. italicum in Japan. Pl. Dis. Reptr 60: 168–172.Google Scholar
  14. Magie, R.O. & Wilfret, G.J., 1974. Tolerance of Fusarium oxysporum f. sp. gladioli to benzimidazole fungicides. Pl. Dis. Reptr 58: 256–259.Google Scholar
  15. McWorther, F.P., 1947. Scale tip rot of Lilium longiflorum in the Pacific Northwest. Pl. Dis. Reptr 31: 159–161.Google Scholar
  16. Meyer, E., 1976. Resistenzbildung gegen systemische Fungizide (Benzimidazol-derivate) bei Colletotrichum lindemuthianum (Sacc. et Magn.) Bri. et Cav. Mitt. biol. BundAnst. Ld- u. Forstw. 166, 135 pp.Google Scholar
  17. Ogilvie, L., 1937. Some experiments with lilies. Lily Yb., Lond. 6: 106–108.Google Scholar
  18. Poeteren, N. van, 1927. Verslag over de werkzaamheden van den Plantenziektenkundigen Dienst in het jaar 1927, Wageningen, p. 32–33.Google Scholar
  19. Poeteren, N. van, 1937. Verslag over de werkzaamheden van den Plantenziektenkundigen Dienst in het jaar 1936, Wageningen, p. 33–34.Google Scholar
  20. Rattink, H., 1974. Jversl. Proefstn Bloemist. Aalsmeer 1973: 82–83.Google Scholar
  21. Richardson, L.T., 1973. Adaptive tolerance of Fusarium solani to benzimidazole derivatives in vitro. Can. J. Bot. 57: 1725–1732.CrossRefGoogle Scholar
  22. Ruppel, E.G., 1975. Biology of benomyl-tolerant strains of Cercospora beticola from sugar beet. Phytopathology 65: 785–789.CrossRefGoogle Scholar
  23. Schenk, P.K. & Boontjes, J., 1970. Lilies in the Netherlands. Lily Yb., Lond. 33: 47–57.Google Scholar
  24. Sheridan, W.F., 1968. Tissue culture of the monocot Lilium. Planta 82: 189–192.CrossRefGoogle Scholar
  25. Tramier, R. & Bettachini, A., 1974. Mise en évidence d’une souche de Fusarium oxysporum f. sp. dianthi résistante aux fongicides systémiques. Annls Phytopath. 6: 231–236.Google Scholar
  26. Tuyl, J.M. van, 1975. Genetic aspects of acquired resistance to benomyl and thiabendazole in a number of fungi. Meded. Fac. Landbouww. Rijksuniv. Gent 40: 691–697.Google Scholar
  27. Valášková, E., 1973. Rezistence některých druhů rodu Fusarium vůči Benlatu. Ochr. Rost. 9: 133–140.Google Scholar
  28. Wit, P.J.G.M. de, 1977. A light and scanning-electron microscopic study of infection of tomato plants by virulent and avirulent races of Cladosporium fulvum. Neth. J. Pl. Path. 83: 109–122.CrossRefGoogle Scholar

Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 1977

Authors and Affiliations

  • G. J. Bollen
    • 1
  1. 1.Laboratory of PhytopathologyAgricultural UniversityWageningenThe Netherlands

Personalised recommendations