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Dilatometric studies in the enzymic hydrolysis of polysaccharides

Part I. Hydrolysis of inulin
  • M. Sreenivasaya
  • H. B. Sreerangachar
  • N. Keshava Iyengar
Article

Summary

  1. 1.

    The kinetics of the enzymic hydrolysis of inulin has been followed in the two-bulbed dilatometer and also by an estimation of the fructose released by Bertrand's method.

     
  2. 2.

    For a given concentration of substrate, inulin hydrolysis is accompanied by a greater depression of volume than that obtained for starch hydrolysis. It is suggested that systems involving the release of fructose during hydrolysis suffer a greater volume depression; this is well illustrated by the difference in volume occurring during the hydrolysis of maltose and sucrose.

     
  3. 3.

    The dilatometric depression per millimol release of fructose during the hydrolysis of inulin is 7·9 mm3 (average value) and is independent of the concentration of the inulin employed. Errors involved in the early stages of the reaction are indicated.

     

Keywords

Fructose Enzymic Hydrolysis Maltose Inulin Great Depression 
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References

  1. Bertrand and Thomas “Guide pour les manipulations de Chimie biologique,” Paris, 1910.Google Scholar
  2. Peter Rona and Nelly Neuenschwander-LemmerBiochem. Z.,235, 214, 1931.Google Scholar
  3. Sreenivasaya and SastriBiochem. J.,23, 975, 1929.PubMedGoogle Scholar
  4. Sreenivasaya, Sastri and SreerangacharBiochem. J.,28, 351, 1934.PubMedGoogle Scholar
  5. Sreenivasaya and SreerangacharNature,128, 585, 1931.CrossRefGoogle Scholar
  6. Do. and do.J. Indian Inst. Sci.,15A, 17, 1932.Google Scholar
  7. Sreerangachar and SreenivasayaJ. Indian Inst. Sci.,16A, 69, 1933.Google Scholar

Copyright information

© Indian Academy of Sciences 1934

Authors and Affiliations

  • M. Sreenivasaya
    • 1
  • H. B. Sreerangachar
    • 1
  • N. Keshava Iyengar
    • 1
  1. 1.Department of BiochemistryIndian Institute of ScienceBangalore

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