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A study on the influence of preoperative radiotherapy to proliferating cell nuclear antigen (PCNA) for oral cancer cells

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Abstract

In order to evalute the effects of preoperative irradiation therapy on oral cancer, monoclonal antibody to proliferating cell nuclear antigen (PCNA) was used to calculate PCNA positive rates before and after 30Gy irradiation therapy. The subjects were 24 patientas with oral cancer. Based on the results, the relation between PCNA positive rate and the histolosical effects of irradiation therapy was quantitatively analyzed. The conclusions are summarized as follows.

  1. 1)

    The average pretreatment PCNA positive rate was 33.4±11.4% while the average posttreatment rate was 24.0±7.9%.

  2. 2)

    Subjects were classified according to the degree of differentiation for pretreatment and posttreatment comparison. However, there existed no significant difference in PCNA positive rate.

  3. 3)

    Subjects were classified according to stage of oral cancer for pretreatment and posttreatment comparsion, however there existed no significant difference in PCNA positive rate.

  4. 4)

    When the decrease in PCNA rate was compared between the group showing negative irradiation effects and that showing positive effects, the fact that the latter group showed remarkably greater decrease in PCNA rate, indicated a significant difference between the two groups.

  5. 5)

    Pretreatment PCNA positive rate was higher in the group showing positive irradiation effects than in that showing negative effects and the difference was significant between the two groups. Beneficial effects of irradiation therapy could be expected in those who showed pretreatment PCNA positive rates of more than 30.0%.

  6. 6)

    The accuracy rate for giagnosis based on PCNA positive rate, and macroscopic and histologic judgment was 58.3%.

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References

  1. Fulwyler MJ: Standards for flow cytometry, Flow cytometry and sorting (M. R. Melamed, et al. eds) John Wiley and Sons. Inc., New York, 1979, 351–358.

    Google Scholar 

  2. Terashima T and Tolamach LJ: Changes in X-ray sensitivirity of HeLa cells during the division cycle, Nature,190: 1210, 1961.

    Article  Google Scholar 

  3. Miyachi K, Fritzler MJ and Tan ENG M: Autoantibody to a nuclear antigen in proliferating cells, J Immunol,121: 2228–2234, 1978.

    PubMed  Google Scholar 

  4. Takasaki Y: A nuclear antigen associated with cell ploliferation and blast transformation. Its distribution in synchronized cells, J Exp Med,159: 911–922, 1984.

    Article  Google Scholar 

  5. Su-Ming, L Raine and Fanger H: Use of Avidin-Biotin-Peroxidase Complex (ABC) in immunoperoxidase techniques, J Histochem Cytochem,29: 577–580, 1981.

    Article  Google Scholar 

  6. Simosato Y, Ohbosi Y and Baba K: Histological evalution of effect of radiotheray and chemotherapy for carcinomas, Jpn J Clin Oncol, 19–35, 1971.

  7. Nisioka A, Ogawa Y and Maeda T: Application of immunohistochemistry for prospective assay in cancer radiotherapy. 2. Special reference to composition of tumor matrix and vascularity, Radiat Syst Res Suppl,5: 225–228, 1988.

    Google Scholar 

  8. Gerdes J, Lemke H, Baisch H, Wacker H, Schwab U, et al.: Cell cycle analysis of a cell roliferation associated human uuclear antigend defined by the monoclonal antibody Ki-67, J Immunol,133: 1710, 1984.

    PubMed  Google Scholar 

  9. Bravo R, Fey SJ, Bellatin J, Larsen PM, Arevalo J, et al.: Identification of a uuclear and of a cytoplasmic polypeptide whose relative propotions are sensitive to changes in the rate of cell proliferation, Exp Cell Res,136: 311, 1981.

    Article  PubMed  Google Scholar 

  10. Bravo R, Frank R, Blundell PA and Macdnald-Bravo H: Cyclin/PCNA is the auxilliary protein of DNA polymerase σ, Natur,326: 515, 1987.

    Article  Google Scholar 

  11. Prelich G, Tan CK and Kostura M: Functional identity of proliferating cell uuclear antigen and a DNA polymerase-σ auxiliiary protein, Nature,326: 517, 1987.

    Article  PubMed  Google Scholar 

  12. Mathews MB, Bernstein RM, Franza Jr. BR and Garres JI: Identity of the proliferation cell uuclear antigen and cyclin, Nature,309: 374, 1984.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery I, The Nippon Dental University School of Dentistry at Tokyo, Tokyo, Japan

    Osamu Munemura, Masashi Tanaka & Masayori Shirakawa

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  1. Osamu Munemura
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  2. Masashi Tanaka
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  3. Masayori Shirakawa
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Munemura, O., Tanaka, M. & Shirakawa, M. A study on the influence of preoperative radiotherapy to proliferating cell nuclear antigen (PCNA) for oral cancer cells. Shigaku = Odontology 86, 121–129 (1998). https://doi.org/10.1007/BF03039151

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  • DOI: https://doi.org/10.1007/BF03039151

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