Journal of Plant Biology

, 51:424 | Cite as

Suppressed Leaf Senescence in Chrysanthemum Transformed with a Mutated Ethylene Receptor GenemDG-ERS1(etr1-4)

  • Shigeru Satoh
  • Masanobu Watanabe
  • Keiko Chisaka
  • Takako Narumi


Previously, Narumi et al. (2005) generated chrysanthemum plants transformed with a mutated ethylene receptor gene (mDG-ERS1(etr1-4<), and showed that thein vitro plantlets of the transformants grown aseptically in a small plastic container had a reduced sensitivity to ethylene resulting in reduced leaf yellowing after exposure to exogenous ethylene. In the present study we evaluated ethylene sensitivity of the transformants using soil-grown mature plants. When the shoots detached from soil-grown plants were treated with exogenous ethylene under continuous light, leaf yellowing (senescence) was delayed in the transformants as compared with the non-transformed plants. Furthermore, when the detached shoots were kept in darkness without ethylene treatment, the transformants showed reduced senescence as compared with those of the non-transformed plants. These results demonstrated that the mutated ethylene receptor genemDG-ERS1(etr1-4) could confer reduced sensitivity to ethylene in the leaves of mature chrysanthemum plants. This gene may be useful to generate transgenicCompositae vegetables with leaves green for a longer time and thus having a longer shelf life.


Chrysanthemum morifolium (Dendranthema grandiflorum) ethylene insensitive leaf deterioration leaf senescence leaf yellowing transgenic chrysanthemum 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Shigeru Satoh
    • 1
    • 3
    • 4
  • Masanobu Watanabe
    • 1
  • Keiko Chisaka
    • 4
  • Takako Narumi
    • 2
  1. 1.Graduate School and Faculty of AgricultureTohoku UniversitySendaiJapan
  2. 2.Faculty of AgricultureKagawa UniversityKagawa PrefectureJapan
  3. 3.Kyoto Prefectural Institute of Agricultural BiotechnologyKyoto PrefectureJapan
  4. 4.Graduate School and Faculty of Life and Environmental SciencesKyoto Prefectural UniversityKyotoJapan

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