, Volume 38, Issue 7, pp 876–877 | Cite as

Absence of effect of culture duration on glucose-activated alterations in intracellular calcium concentration in mouse pancreatic islets

  • M. W. Roe
  • B. Spencer
  • M. E. Lancaster
  • R. J. Mertz
  • J. F. Worley
  • I. D. Dukes
Letters to the editor


Mouse Islet NMRI Mouse Culture Duration Endoplasmic Reticulum Calcium Mouse Pancreatic Islet 
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  1. 1.
    Gilon P, Jonas JC, Henquin JC (1994) Culture duration and conditions affect the oscillations of cytoplasmic calcium concentration induced by glucose in mouse pancreatic islets. Diabetologia 37: 1007–1014PubMedCrossRefGoogle Scholar
  2. 2.
    Prentki M, Matschinsky FM (1987) Ca2+, cAMP, and phos-pholipid-derived messengers in coupling mechanisms of insulin secretion. Physiol Rev 67: 1185–1248PubMedGoogle Scholar
  3. 3.
    Roe MW, Lancaster ME, Mertz RJ, Worley JF III, Dukes ID (1993) Voltage-dependent intracellular calcium release from mouse islets stimulated by glucose. J Biol Chem 268: 9953–9956PubMedGoogle Scholar
  4. 4.
    Gylfe E (1988) Glucose-induced early changes in cytoplasmic calcium of pancreatic β -cells studied with time-sharing dual-wavelength fluorometry. J Biol Chem 263: 5044–5048PubMedGoogle Scholar
  5. 5.
    Roe MW, Mertz RJ, Lancaster ME, Worley JF III, Dukes ID (1994) Thapsigargin inhibits the glucose-induced decrease of intracellular Ca2+ in mouse islets of Langerhans. Am J Physiol 266: E852-E862PubMedGoogle Scholar
  6. 6.
    Rojas E, Carroll PB, Ricordi C, Boschero AC, Stojilkovic SS, Atwater I (1994) Control of cytosolic free calcium in cultured human pancreatic beta-cells occurs by external calcium-dependent and independent mechanisms. Endocrinology 134: 1771–1781PubMedCrossRefGoogle Scholar
  7. 7.
    Worley JF III, Mclntyre MS, Spencer B, Mertz RJ, Roe MW, Dukes ID (1994) Endoplasmic reticulum calcium store regulates membrane potential in mouse islet β -cells. J Biol Chem 269: 14359–14362PubMedGoogle Scholar
  8. 8.
    Worley JF III, Mclntyre MS, Spencer B, Dukes ID (1994) Depletion of intracellular Ca2+ stores activates a maitotox-in-sensitive non-selective cationic current in β -cells. J Biol Chem 269: 32055–32058PubMedGoogle Scholar
  9. 9.
    Ashcroft FM, Rorsman P (1989) Electrophysiology of the pancreatic β -cell. Prog Biophys Mol Biol 54: 87–143PubMedCrossRefGoogle Scholar
  10. 10.
    Gilon P, Henquin JC (1992) Influence of membrane potential changes on cytoplasmic Ca2+ concentration in an electrically excitable cell, the insulin-secreting pancreatic B cell. J Biol Chem 268: 9314–9319Google Scholar
  11. 11.
    Dukes ID, Cleemann L (1993) Calcium current regulation of depolarization-evoked calcium transients in β-cells (HIT-T15). Am J Physiol 264: E348-E353PubMedGoogle Scholar
  12. 12.
    Dukes ID, Mclntyre MS, Mertz RJ, et al. (1994) Dependence on NADH produced during glycolysis for β-cell glucose signaling. J Biol Chem 269: 10979–10982PubMedGoogle Scholar
  13. 13.
    Eizirik DL, Strandeil E, Sandler S (1991) Prolonged exposure of pancreatic islets isolated from “pre-diabetic” nonobese diabetic mice to a high glucose concentration does not impair beta-cell function. Diabetologia 34: 6–11PubMedCrossRefGoogle Scholar
  14. 14.
    Roe MW, Philipson LH, Frangakis CJ et al. (1994) Defective glucose-dependent endoplasmic reticulum Ca2+ sequestration in diabetic mouse islets of Langerhans. J Biol Chem 269: 18279–18282PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • M. W. Roe
    • 1
  • B. Spencer
    • 1
  • M. E. Lancaster
    • 1
  • R. J. Mertz
    • 1
  • J. F. Worley
    • 1
  • I. D. Dukes
    • 1
  1. 1.Department of Cell PhysiologyGlaxo Research Institute ResearchUSA

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