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Neurotoxicity Research

, Volume 9, Issue 2–3, pp 93–100 | Cite as

Characterization ofClostridial botulinum neurotoxin channels in neuroblastoma cells

  • Audrey Fischer
  • Mauricio Montal
Article

Abstract

The channel and chaperone activities ofClostridial botulinum neurotoxin (BoNT) A were investigated in Neuro 2a neuroblastoma cells under conditions that closely emulate those prevalent at the endosome. Channel activity occurs in bursts interspersed between periods of little or no activity. The channels are voltage dependent, opening only at negative voltages. Within bursts, the channel resides preferentially in the open state. The channels open to a main conductance of 105 ± 5 pS or 65 ± 4 pS in 200 mM CsCl or NaCl, respectively. The BoNT channels display a conspicuous subconductance of 10 ± 2 pS. The neuroblastoma cell line appears, therefore, to be a suitable system to characterize the BoNT channel and to pursue evaluation of plausible strategies for targeted drug delivery thereby minimizing the requirement forin vivo animal testing.

Keywords

Botulinum neurotoxin Channels Chaperones Targeted drug screen Protein translocation 

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Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Section of Neurobiology, Division of Biological SciencesUniversity of California San DiegoLa JollaUSA

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