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Investigation of microsatellite instability in Turkish breast cancer patients

  • Semra Demokan
  • Mahmut Muslumanoglu
  • Yazici H. 
  • Abdullah Igci
  • Nejat Dalay
Article

Abstract

Multiple somatic and inherited genetic changes that lead to loss of growth control may contribute to the development of breast cancer. Microsatellites are tandem repeats of simple sequences that occur abundantly and at random throughout most eucaryotic genomes. Microsatellite instability (MI), characterized by the presence of random contractions or expansions in the length of simple sequence repeats or microsatellites, is observed in a variety of tumors. The aim of this study was to compare tumor DNA fingerprints with constitutional DNA fingerprints to investigate changes specific to breast cancer and evaluate its correlation with clinical characteristics. Tumor and normal tissue samples of 38 patients with breast cancer were investigated by comparing PCR-amplified microsatellite sequences D2S443 and D21S1436. Microsatellite instability at D21S1436 and D2S443 was found in 5 (13%) and 7 (18%) patients, respectively. Two patients displayed instability at both marker loci. No association was found between MI and age, family history, lymph node involvement and other clinical parameters.

Keywords

microsatellite instability breast cancer 

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Copyright information

© Arányi Lajos Foundation 2002

Authors and Affiliations

  • Semra Demokan
    • 1
  • Mahmut Muslumanoglu
    • 2
  • Yazici H. 
    • 1
  • Abdullah Igci
    • 2
  • Nejat Dalay
    • 1
  1. 1.Department of Basic OncologyOncology InstituteTurkey
  2. 2.Istanbul Faculty of Medicine, Department of SurgeryIstanbul UniversityIstanbulTurkey

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