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Neurotoxicity Research

, Volume 13, Issue 3–4, pp 241–246 | Cite as

Effects of sertoli cell-conditioned medium on ventral midbrain neural stem cells: A preliminary report

  • Rania Shamekh
  • Samuel Saporta
  • Don F. Cameron
  • Alison E. Willing
  • Cindy D. Sanberg
  • Karl Johe
  • P. R. Sanberg
Article

Abstract

The 796RMB cell line is a multipotent stem cell line isolated from human fetal midbrain tissues, a region from which dopamine neurons of the substantia nigra develop. It would be useful to increase the dopaminergic characteristics of this cell line to enhance its usefulness as a cell therapy for Parkinson’s disease utilizing transplantation protocols. Sertoli cells and its conditioned media isolated from the testis have been previously shown to enhance tyrosine hydroxylase expression in ventral mesencephalon neurons bothin vitro andin vivo. Therefore, the present preliminary study investigated the ability of Sertoli cell pre-conditioned medium to enhance differentiation of the 796MB cell line toward the domaminergic phenotype. Results showed that secretory products derived from Sertoli cell conditioned medium increased cell proliferation and enhanced dopaminergic neuronal differentiation of the 796RMB cell line. These findings may lead to alternative therapeutic cell transplantation protocols for the treatment of Parkinson’s disease.

Keywords

Sertoli cells Stem cells Parkinson’s disease Fetal tissue Growth factors 

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Copyright information

© Springer 2008

Authors and Affiliations

  • Rania Shamekh
    • 1
  • Samuel Saporta
    • 2
    • 3
  • Don F. Cameron
    • 2
  • Alison E. Willing
    • 1
    • 2
    • 3
  • Cindy D. Sanberg
    • 4
  • Karl Johe
    • 5
  • P. R. Sanberg
    • 1
    • 3
  1. 1.Center of Excellence for Aging & Brain Repair, Department of NeurosurgeryUniversity of South Florida College of MedicineTampa
  2. 2.Center of Excellence for Aging & Brain Repair, Department of Pathology and Cell BiologyUniversity of South Florida College of MedicineTampa
  3. 3.Center of Excellence for Aging & Brain Repair, Department of MolecularPharmacology and PhysiologyUniversity of South Florida College of MedicineTampa
  4. 4.Center of Excellence for Aging & Brain Repair, Department of Saneron CCEL TherapeuticsUSF Center for EntrepreneurshipTampa
  5. 5.NEURALSTEM, Inc.RockvilleUSA

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