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Expression of a decorin-like molecule in human melanoma

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Pathology & Oncology Research

Abstract

Decorin, a member of the family of small leucin-rich proteoglycans, has originally been described as a secreted proteoglycan component of the connective tissues, and has been implicated in the negative regulation of cell proliferation directly or via interactions with TGF-β. It was reported to be generally absent from tumor cells. Here we show that human melanoma cell lines express a decorin-like molecule. We detected decorin mRNA by RT-PCR in 7 out 7 human melanoma lines characterized by various metastatic potential. Using polyclonal antiserum against the core protein of decorin, the typical 80–120 kD glycanated form as well as a high molecular weight aberrant version (200–210 kD) of decorin were demonstrated by Western blot technique in the culture supernatants as well as in lysates of human melanoma cells. Finally, decorin epitope was also demonstrated immunohistochemically in human melanoma xenografts, as well as in tumor cells of surgically resected melanomas but not in melanocytes of nevi. The expression of this aberrant decorin did not inhibit thein vitro orin vivo growth of human melanoma cells, and it was independent of their metastatic potential. Human melanoma cell lines expressing aberrant decorin retained sensitivity to the antiproliferative and gelatinase-stimulatory effects of exogenous TGF-β.

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Abbreviations

CS:

chondroitin sulfate

PG:

proteoglycan

TGF:

transforming growth factor

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Authors and Affiliations

  1. Department of Tumor Progression, National Institute of Oncology, Ráth Gy. u.7-9., H-1122, Budapest, Hungary

    Andrea Ladányi & József Tímár

  2. Department of Molecular Pathology, National Institute of Oncology, Budapest

    Mónika Gallai

  3. Department of Molecular Pathology, Joint Organization of the Hungarian Academy of Sciences and Semmelweis University, Hungary

    Sándor Paku

  4. 1st Institute of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary

    Julianna O. Nagy, József Dudás, József Tímár & Ilona Kovalszky

Authors
  1. Andrea Ladányi
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  2. Mónika Gallai
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  3. Sándor Paku
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  4. Julianna O. Nagy
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  5. József Dudás
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  6. József Tímár
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  7. Ilona Kovalszky
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Correspondence to József Tímár.

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Ladányi, A., Gallai, M., Paku, S. et al. Expression of a decorin-like molecule in human melanoma. Pathol. Oncol. Res. 7, 260–266 (2001). https://doi.org/10.1007/BF03032382

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  • DOI: https://doi.org/10.1007/BF03032382

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