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Journal of Plant Biology

, Volume 49, Issue 4, pp 281–285 | Cite as

Recombinant AtNF-YA, a subunit of theArabidopsis CCAAT-binding transcription factor, forms a protein-DNA complex with mammalian NF-YB and NF-YC

  • Soomin Park
Article

Abstract

The evolutionary conserved CCAAT binding protein NF-Y is a common regulatory DNA binding protein consisting of three distinct subunits. Unlike yeast and mammals, in which only a single copy of each subunit is encoded,Arabidopsis encodes a multi-gene family for each subunit in its genome. Compared with the NF-Y of mammals or yeast, very little is known about plant NF-Y homologs. HereArabidopsis NF-YA subunits were isolated to determine whether they could form a hete-rotrimeric NF-Y complex with mammalian NF-YB and NF-YC. This resultant chimeric NF-Y complex had DNA binding ability to the same CCAAT sequences as those of the other life systems. Therefore, it is possible that plant NF-Y homologs might have biochemical characteristics similar to mammalian NF-Y, thereby suggesting its functional conservation among organisms.

Keywords

Arabidopsis genome electrophoretic mobility shift assay Nuclear Factor Y yeast three-hybrid assay 

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Copyright information

© The Botanical Society of Korea 2006

Authors and Affiliations

  1. 1.Section of Plant Biology, Division of Biological SciencesUniversity of CaliforniaUSA

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