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Journal of Plant Biology

, Volume 42, Issue 1, pp 23–31 | Cite as

Molecular cloning and characterization of constans-like cDNA clones of the fuji apple

  • Dong-Hoon Jeong
  • Soon-Kee Sung
  • Gynheung An
Article

Abstract

Two cDNA clones,MdCOL1 andMdCOL2, encoding CONSTANS (CO)-like proteins were isolated from an apple (Malus domestica cv. Fuji) fruit cDNA library. Both proteins contain two zinc finger motifs at the amino terminal end and a putative nuclear localization domain at the carboxyl terminal end. Genomic DNA blot analysis suggests that theCO-like genes are members of a small multigene family. RNA blot and RT-PCR analyses revealed that these genes are expressed in every organ that was examined. However, the expression levels were higher in floral buds and fruits at their early developmental stages compared to late reproductive stages or vegetative organs. Such expression patterns are quite different from those of theCO-like genes fromArabidopsis, which show strong organ specificity in either roots, cauline leaves, or flowers. These results indicate that the appleCO-like genes are significantly different from theArabidopsis genes and that they appear to play important roles in reproductive organ development.

Keywords

apple CONSTANS development fruit zinc finger motifs 

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Copyright information

© The Botanical Society of Korea 1999

Authors and Affiliations

  1. 1.Department of Life SciencePohang University of Science and TechnologyPohangKorea

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